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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Shewanella sediminis (strain HAW-EB3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi98ZincUniRule annotation1
Metal bindingi100ZincUniRule annotation1
Metal bindingi125ZincUniRule annotation1
Metal bindingi127ZincUniRule annotation1
Binding sitei239ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSED425104:G1G9Y-3103-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Ssed_2906
OrganismiShewanella sediminis (strain HAW-EB3)
Taxonomic identifieri425104 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000002015 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000743351 – 469Glutamate--tRNA ligaseAdd BLAST469

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi425104.Ssed_2906

Structurei

3D structure databases

ProteinModelPortaliA8FXE0
SMRiA8FXE0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 19"HIGH" regionUniRule annotationAdd BLAST11
Motifi236 – 240"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A8FXE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTKTRFAPS PTGFLHVGGA RTALYSWLYA RANQGEFVLR VEDTDIERST
60 70 80 90 100
PEACEAILEG MQWLGLNWDE GPYYQTKRFD RYNEIIAQML EQGTAYKCYC
110 120 130 140 150
SRERIETMRD EQAAKGEQQK YDGCCRDKAP RDTDEPFVIR FKNPTEGSVV
160 170 180 190 200
FDDHVRGRIE ISNDLLDDLI IARTEGTPTY NFCVVVDDWD MGITCVVRGE
210 220 230 240 250
DHINNTPRQI NILKALGAPI PEYAHVAMIL GDDGAKLSKR HGAVGVMQYR
260 270 280 290 300
DDGYLPEALL NYLVRLGWSH GDQEVFSIDE MKQLFKLDDI NKAASAFNTE
310 320 330 340 350
KLNWLNQHYI KELDPEYVAK HLEWHMADQK IDTSNGPALS AVVTALSERA
360 370 380 390 400
KTLKELAASS RYFYEDFAEF DATAAKKHLR GVAMEPLELV QKKLAELSEW
410 420 430 440 450
TLEGIHQAIE DTATELEVGM GKVGMPLRVA VTGAGMSPAV DLTLFLVGKA
460
RCEQRISKAI EFVANRINS
Length:469
Mass (Da):52,865
Last modified:November 13, 2007 - v1
Checksum:iF546409068E927C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000821 Genomic DNA Translation: ABV37513.1
RefSeqiWP_012143243.1, NC_009831.1

Genome annotation databases

EnsemblBacteriaiABV37513; ABV37513; Ssed_2906
KEGGisse:Ssed_2906

Similar proteinsi

Entry informationi

Entry nameiSYE_SHESH
AccessioniPrimary (citable) accession number: A8FXE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 13, 2007
Last modified: March 28, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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