Reviewed, UniProtKB/Swiss-Prot A8FVR6 (ASTE_SHESH)

Names and origin

Protein names

Recommended name:
    Succinylglutamate desuccinylase
    EC=3.5.1.96

Gene names

Name:	astE
Ordered Locus Names:	Ssed_2330

Organism

Shewanella sediminis (strain HAW-EB3) [Complete proteome] [HAMAP]

Taxonomic identifier

425104 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transforms N(2)-succinylglutamate into succinate and glutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP MF_00767
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP MF_00767
Sequence similarities	Belongs to the aspA/astE family. Succinylglutamate desuccinylase subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: HAMAP succinylglutamate desuccinylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Succinylglutamate desuccinylase HAMAP MF_00767		PRO_1000083550
Sites
	Active site	224	1	Potential
	Metal binding	63	1	Zinc By similarity
	Metal binding	66	1	Zinc By similarity
	Metal binding	160	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FVR6-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 39A3FA0174F1ECF8	FASTA	347	39,324
	10 20 30 40 50 60 MFQALKESKD FLNLTLAHPQ HIDETCSFSV GDHTQVEVWD TGVIVFEPKV NQAKDIVLSC 70 80 90 100 110 120 AVHGNETAPI ELCNELVTAL LEEKLIAKQR VLFLFGNPAA IINGTRFIDE NLNRLFNGAH 130 140 150 160 170 180 SSGEGLVNPE RIRAKKLEFY VDKFFAGQRE ERHRIHYDLH TAIRGSKHEK FAIYPYRPGR 190 200 210 220 230 240 KFSGEQIMFL EACGIDTVLF HHEPTTTFSY FSSLNYQADA FTIELGKVLP MGQNDMTRFI 250 260 270 280 290 300 ALKEMLNRLI GNDPLELSAF DVNKVNLYQV CRAINKEFDD FEFTFTNDVE NFTSFPEGYI 310 320 330 340 LAKEGGNNIK VEHKVEAIVF PNAKVPVGQR TVLCLKPATT ENIDLGN

References

[1]

"Complete sequence of Shewanella sediminis HAW-EB3." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Zhao J.-S., Richardson P. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000821 Genomic DNA. Translation: ABV36939.1.
RefSeq	YP_001474067.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5611259.
GenomeReviews	Gene locus Ssed_2330 in contig CP000821_GR.
KEGG	sse:Ssed_2330.
NMPDR	fig|425104.3.peg.2066.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8FVR6. EKFAIYP.
Family and domain databases
HAMAP	MF_00767. [Tree]
InterPro	IPR007036. Aste_AspA. IPR016681. SuccinylGlu_desuccinylase. [Graphical view]
Pfam	PF04952. AstE_AspA. 1 hit. [Graphical view]
PIRSF	PIRSF017020. AstE. 1 hit.
TIGRFAMs	TIGR03242. arg_catab_astE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTE_SHESH

Accession

Primary (citable) accession number: A8FVR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 13, 2007
Last modified:	June 16, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)