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A8FUV3

- THIG_SHESH

UniProt

A8FUV3 - THIG_SHESH

Protein

Thiazole synthase

Gene

thiG

Organism
Shewanella sediminis (strain HAW-EB3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

    Catalytic activityi

    1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei95 – 951Schiff-base intermediate with DXPUniRule annotation
    Binding sitei156 – 1561DXP; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. 2-iminoacetate synthase activity Source: InterPro
    2. sulfurtransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    BioCyciSSED425104:GH7Q-2081-MONOMER.
    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
    Gene namesi
    Name:thiGUniRule annotation
    Ordered Locus Names:Ssed_2017
    OrganismiShewanella sediminis (strain HAW-EB3)
    Taxonomic identifieri425104 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000002015: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 254254Thiazole synthasePRO_1000080880Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

    Protein-protein interaction databases

    STRINGi425104.Ssed_2017.

    Structurei

    3D structure databases

    ProteinModelPortaliA8FUV3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni182 – 1832DXP bindingUniRule annotation
    Regioni204 – 2052DXP bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the ThiG family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2022.
    HOGENOMiHOG000248049.
    KOiK03149.
    OMAiGSQLVTM.
    OrthoDBiEOG6KMBD9.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00443. ThiG.
    InterProiIPR013785. Aldolase_TIM.
    IPR008867. ThiG.
    [Graphical view]
    PfamiPF05690. ThiG. 1 hit.
    [Graphical view]
    SUPFAMiSSF110399. SSF110399. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A8FUV3-1 [UniParc]FASTAAdd to Basket

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    MLKIAEHEFN SRLFTGTGKF SSANLMLEAI QASQSQLVTM AMKRLDLSSG    50
    SDEILLPLQQ SGIKLLPNTS GARNAKEAVF AAELAREVLN TNWVKLEIHP 100
    DPKYLMPDPI ETLAAAKTLC DKGFVVLPYV HADPVLCRRL EEVGCAAVMP 150
    LASPIGSNQG LATETFLKII IEQASVPVVV DAGIGTPSQA THAMELGADA 200
    VLVNTAIASS RDPIAMARCF SQAVETGRAA YRAGLGQVSV RAEHTSPLTG 250
    FLNE 254
    Length:254
    Mass (Da):27,054
    Last modified:November 13, 2007 - v1
    Checksum:iD63C92FD77A6EFE7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000821 Genomic DNA. Translation: ABV36626.1.
    RefSeqiWP_012142361.1. NC_009831.1.
    YP_001473754.1. NC_009831.1.

    Genome annotation databases

    EnsemblBacteriaiABV36626; ABV36626; Ssed_2017.
    GeneIDi5613839.
    KEGGisse:Ssed_2017.
    PATRICi23561542. VBISheSed62411_2145.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000821 Genomic DNA. Translation: ABV36626.1 .
    RefSeqi WP_012142361.1. NC_009831.1.
    YP_001473754.1. NC_009831.1.

    3D structure databases

    ProteinModelPortali A8FUV3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 425104.Ssed_2017.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV36626 ; ABV36626 ; Ssed_2017 .
    GeneIDi 5613839.
    KEGGi sse:Ssed_2017.
    PATRICi 23561542. VBISheSed62411_2145.

    Phylogenomic databases

    eggNOGi COG2022.
    HOGENOMi HOG000248049.
    KOi K03149.
    OMAi GSQLVTM.
    OrthoDBi EOG6KMBD9.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .
    BioCyci SSED425104:GH7Q-2081-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00443. ThiG.
    InterProi IPR013785. Aldolase_TIM.
    IPR008867. ThiG.
    [Graphical view ]
    Pfami PF05690. ThiG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF110399. SSF110399. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HAW-EB3.

    Entry informationi

    Entry nameiTHIG_SHESH
    AccessioniPrimary (citable) accession number: A8FUV3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3