A8FQ89 (GLPK_SHESH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Ssed_0399

Organism

Shewanella sediminis (strain HAW-EB3) [Complete proteome] [HAMAP]

Taxonomic identifier

425104 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism By similarity. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	Glycerol kinase HAMAP MF_00186		PRO_1000077431
Regions
	Nucleotide binding	410 – 414	5	ATP By similarity
Sites
	Binding site	13	1	Substrate By similarity
	Binding site	17	1	ATP By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	266	1	ATP By similarity
	Binding site	309	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FQ89 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: E69A88331F74860D	FASTA	495	54,105
	10 20 30 40 50 60 MAKKYVVALD QGTTSSRAII FDHDANIVSV SQREFPQIYP QAGWVEHDPM EIWASQSSTL 70 80 90 100 110 120 IELLARSGIH GSEVAAIGIT NQRETTVIWD KLTGKPVYNA IVWQCRRSSH ICDELKAQGL 130 140 150 160 170 180 EDYVRETTGL LLDPYFSGTK IKWILDNVAG VRERAEKGEL LFGTIDTWLV WKLTEGKVHV 190 200 210 220 230 240 TDPTNASRTL LFNIHTQQWD SKLLEALNIP ESLLPEVKPS CAVYGKTRIA GEGGEISIAG 250 260 270 280 290 300 IAGDQQSALF GQLCIDEGMA KNTYGTGCFL LMNTGKQAVK STHGLLTTVA IGADCEVNYA 310 320 330 340 350 360 LEGAVFMGGA TIQWLRDELG LIRDAQDTEY FASKVEDTNG VYLVPAFVGL GAPYWDPNAR 370 380 390 400 410 420 GALVGLTRGS NRNHIIRAAL EAIAYQSRDL LDAMAKDSGV MLKQLKVDGG AVSNDFLMQF 430 440 450 460 470 480 QADITNVEVQ RPAITETTAM GAAFLAGLAV GFWSSTSELK HKADIERTFI PSISAEKCDE 490 LYCGWNRAVT QTIKS

References

[1]

"Complete sequence of Shewanella sediminis HAW-EB3." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Zhao J.-S., Richardson P. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HAW-EB3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000821 Genomic DNA. Translation: ABV35012.1.
RefSeq	YP_001472140.1. NC_009831.1.
3D structure databases
ProteinModelPortal	A8FQ89.
SMR	A8FQ89. Positions 3-493.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FQ89.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5612394.
GenomeReviews	Gene locus Ssed_0399 in contig CP000821_GR.
KEGG	sse:Ssed_0399.
NMPDR	fig|425104.3.peg.352.
PATRIC	23557970. VBISheSed62411_0414.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HBG511469.
OMA	SIAYQSN.
ProtClustDB	CLSK907562.
Enzyme and pathway databases
BioCyc	SSED425104:SSED_0399-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_SHESH

Accession

Primary (citable) accession number: A8FQ89

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program