Reviewed, UniProtKB/Swiss-Prot A8FNJ7 (ACSA_CAMJ8)

Names and origin

Protein names

Recommended name:
    Acetyl-coenzyme A synthetase
    EC=6.2.1.1
Alternative name(s):
    Acetate--CoA ligase
    Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	C8J_1436

Organism

Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) [Complete proteome] [HAMAP]

Taxonomic identifier

407148 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 657	657	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000073043
Sites
	Active site	521	1	By similarity
Amino acid modifications
	Modified residue	617	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FNJ7-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 5F0EB3B36C18B459	FASTA	657	73,824
	10 20 30 40 50 60 MLNQNNQELF KPSKEFSRNA RIKNLCEYYD LCDEAKEDFE GFWKRQALEK IEWFSPFSRV 70 80 90 100 110 120 LNEDKAPFYK WFEGGTLNVS YQCLDRHMKT RRNKAALIFE GEMGDYEVYT YRRLLHETCK 130 140 150 160 170 180 AANLLKKFGV KKGDRVVIYM PMIPETAIVM LACARIGAIH SVVFGGFSPE ALRDRIIDAG 190 200 210 220 230 240 AKLVVTADGA FRRGKPYMLK PAVDKALSEG CESVEKVLIV IRNNEPIEYI KGRDYVYNEL 250 260 270 280 290 300 VKNESYKCEP EIMDSEDLLF LLYTSGSTGK PKGVMHASAG YILWAQMTME WVFDIKDYDN 310 320 330 340 350 360 YWCSADVGWI TGHTYVVYGP LACGATTIMH EGTPTYPNSG RWWRMIEEYQ ISKFYTSPTA 370 380 390 400 410 420 IRMLHADAPD EPRKYDLSTL EVLGTVGEPI NPSAWKWFYD EIGGTKSPIV DTWWQTETGG 430 440 450 460 470 480 HMITPLPGAT PLKPGCATLP LPGIFAEVID EEGNKKDEGE DGLLCITKPW PSMIRGIWGN 490 500 510 520 530 540 DERYIESYFS QAKKDGKAVY FSGDGAFYDK NGYITITGRT DDVVNVAGHR IGTAEIESAI 550 560 570 580 590 600 AKHPSVAESA VVSILDAIKG ESLFAFVVLS PASSCDLGGA IETLKELNDI LRVEIGPIAK 610 620 630 640 650 IEKILYTPGL PKTRSGKIMR RILRTIARGE EIKQDISTLE DSGVVETIVK LAKAEFE

References

[1]	"The complete genome sequence of Campylobacter jejuni strain 81116 (NCTC11828)." Pearson B.M., Gaskin D.J.H., Segers R.P.A.M., Wells J.M., Nuijten P.J.M., van Vliet A.H.M. J. Bacteriol. 189:8402-8403(2007) [PubMed: 17873037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000814 Genomic DNA. Translation: ABV53034.1.
RefSeq	YP_001483011.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8FNJ7.
Genome annotation databases
GeneID	5618493.
GenomeReviews	Gene locus C8J_1436 in contig CP000814_GR.
KEGG	cju:C8J_1436.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TGGYNLY.
Family and domain databases
HAMAP	MF_01123. [Tree]
InterPro	IPR011904. Ac_CoA_lig_AcsA. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_CAMJ8

Accession

Primary (citable) accession number: A8FNJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)