A8FN24 (DXR_CAMJ8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Short name=DXP reductoisomerase
EC=1.1.1.267

Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomerase
2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	C8J_1262

Organism

Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) [Complete proteome] [HAMAP]

Taxonomic identifier

407148 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00183
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity. HAMAP MF_00183
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: EC NADPH binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_1000071665
Regions
	Nucleotide binding	4 – 30	27	NADP By similarity
Sites
	Metal binding	131	1	Divalent metal cation By similarity
	Metal binding	133	1	Divalent metal cation By similarity
	Metal binding	200	1	Divalent metal cation By similarity
	Binding site	112	1	Substrate By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	200	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FN24 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 6F38F46C1801C811	FASTA	356	39,594
	10 20 30 40 50 60 MILFGSTGSI GVNALKLAAL KNIPISALAC GDNIALLNEQ IARFKPKFVA IKDSKNKHLV 70 80 90 100 110 120 KHDRVFIGQE GLEQILTECQ DKLLFNAIVG FAGLKSTLKA KELGKNIALA NKESLVVAGS 130 140 150 160 170 180 FLKGVKFLPV DSEHAALKFL LEGKKNIAKL YITASGGAFY RYKIKDLNQV SVKDALKHPN 190 200 210 220 230 240 WNMGAKITID SATMANKLFE IIEAYHLYDF KEIDALIEPR SLVHAMCEFK NGASTAYFSK 250 260 270 280 290 300 ADMKLAISDA IFEKQDTPIL EAVDFSKMPA LKFHPISTKK YPIFKLKNTF LKEPNLGVII 310 320 330 340 350 NAANEVGVYN FLENKSGFLD IAKCIFKALD HFGVPKISSI EEVFEYDFKT REYLRS

References

[1]

"The complete genome sequence of Campylobacter jejuni strain 81116 (NCTC11828)." Pearson B.M., Gaskin D.J.H., Segers R.P.A.M., Wells J.M., Nuijten P.J.M., van Vliet A.H.M. J. Bacteriol. 189:8402-8403(2007) [PubMed: 17873037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 81116 / NCTC 11828.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000814 Genomic DNA. Translation: ABV52861.1.
RefSeq	YP_001482838.1. NC_009839.1.
3D structure databases
ProteinModelPortal	A8FN24.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FN24.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5618267.
GenomeReviews	Gene locus C8J_1262 in contig CP000814_GR.
KEGG	cju:C8J_1262.
PATRIC	20056162. VBICamJej119085_1272.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0743.
HOGENOM	HBG430762.
OMA	IAKLYIT.
ProtClustDB	PRK05447.
Enzyme and pathway databases
BioCyc	CJEJ407148:C8J_1262-MONOMER.
Family and domain databases
HAMAP	MF_00183. DXP_reductoisom. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00099.
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_CAMJ8

Accession

Primary (citable) accession number: A8FN24

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program