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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei123S-adenosyl-L-methionineUniRule annotation1
Binding sitei148S-adenosyl-L-methionineUniRule annotation1
Binding sitei175S-adenosyl-L-methionineUniRule annotation1
Binding sitei201SubstrateUniRule annotation1
Binding sitei231SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:C8J_1222
OrganismiCampylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828)
Taxonomic identifieri407148 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000729961 – 392tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST392

Structurei

3D structure databases

ProteinModelPortaliA8FMY4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252163.
KOiK03439.
OMAiHFPVPWN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8FMY4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNFKSKKIK EINLPYSKDD VEFLWLAKND NVSLIYTKVQ EESFFLQIKK
60 70 80 90 100
AQNGFVIKGD KHTKPSKIGY LQKALKIFKE GFCEDIINEA FGLKNNALIE
110 120 130 140 150
KTPFIVDNFD ELLSKLQGKI YIEIGFGSGR HLLYQAKENP NVLILGVEIY
160 170 180 190 200
NPALTQVAKL AKVQNVNNIL LIQSDARLLL SVLKSKSVEK IFLHFPVPWD
210 220 230 240 250
KKPHRRVIGK DFCKECARVL TQNGRFELRT DSFEYFNFTL EQFLTFPAPK
260 270 280 290 300
FSLRKNENLE ISSKYEDRWK KQEKNIYDLW VWNFNQECKN YELNEFNLSS
310 320 330 340 350
VEFSKEDLKK IEQNFKNITI KKDDFFLHFE SIYKQDENLL LKVAFGAFNK
360 370 380 390
PEHCYLHLDK TIDFVFKEPF KIQENIKAIN ELKEILKVQF KI
Length:392
Mass (Da):46,215
Last modified:November 13, 2007 - v1
Checksum:iCE2BE0CBE8DDD2DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000814 Genomic DNA. Translation: ABV52821.1.
RefSeqiWP_002856145.1. NC_009839.1.

Genome annotation databases

EnsemblBacteriaiABV52821; ABV52821; C8J_1222.
KEGGicju:C8J_1222.
PATRICi20056078. VBICamJej119085_1232.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000814 Genomic DNA. Translation: ABV52821.1.
RefSeqiWP_002856145.1. NC_009839.1.

3D structure databases

ProteinModelPortaliA8FMY4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV52821; ABV52821; C8J_1222.
KEGGicju:C8J_1222.
PATRICi20056078. VBICamJej119085_1232.

Phylogenomic databases

HOGENOMiHOG000252163.
KOiK03439.
OMAiHFPVPWN.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_CAMJ8
AccessioniPrimary (citable) accession number: A8FMY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 13, 2007
Last modified: November 2, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.