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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei582 – 5821ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCJEJ407148:GHCS-1067-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:C8J_1032
OrganismiCampylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828)
Taxonomic identifieri407148 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000002013 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 809809Leucine--tRNA ligasePRO_1000071109Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi407148.C8J_1032.

Structurei

3D structure databases

ProteinModelPortaliA8FME4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5011"HIGH" regionAdd
BLAST
Motifi579 – 5835"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8FME4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYEASLIEK KWQKIWDENE YFEPKDDLNL PKKYILSMFP YPSGRIHMGH
60 70 80 90 100
VRNYTIGDAL ARYYRKIGFN VLHPIGFDSF GMPAENAAIK HKIHPKSWTY
110 120 130 140 150
ENIAYMKKEL FSLGFSFSKK RMLATSDPLY TKFEQEFFIK MFEKGLIYTK
160 170 180 190 200
EANVNWCEQD QTVLANEQVE DGKCWRCGHE VVQKKMPGYY VKITAYAEEL
210 220 230 240 250
LKDLEELKDK WPNQVLTMQE NWIGKSEGLE FSLNLDEESK QKTKESSLEV
260 270 280 290 300
FTTRADTIYG VSYIALAPEH KIVQNLLSQN LLNQDVLNKI KAIQNQSPRE
310 320 330 340 350
RQSSEKEGYF LGIYAIHPLS GEKIPLWVAN FVLADYGSGA VMAVPAHDER
360 370 380 390 400
DFEFATKYNL AIKQVIQTQE NLPYTQKSGK LIHSQEFDNL DCNEARLKII
410 420 430 440 450
SQFEAKNIGK RVVNFKIRDW GVSRQRYWGA PIPMIKCQSC GIVPQKLENL
460 470 480 490 500
PITLPEDVQI TGEGNPLDKH PTWKNCICPK CGKEAQKESD TLDTFFESSW
510 520 530 540 550
YFARFASDEK TWQEKALDEK SVKYWMSVDQ YIGGIEHAIL HLLYARFFQK
560 570 580 590 600
ALRDLGYLTQ NEPFDRLLTQ GMVLKDGAKM SKSKGNVVDP DEIIEKYGAD
610 620 630 640 650
TARLFILFAA PPAKELEWND DAVEGAYRFI CKLYDRAQNV KKGELVELKQ
660 670 680 690 700
ENLNKEEKYA RLKVYEALKK SFEVYHQSFA FNTLIAACME ALNALALCKN
710 720 730 740 750
EALEQEAFYI ILNILEPIIP HVCFELSEEL FKCKNFKKLE LKEEIFVKDT
760 770 780 790 800
LNLAVSINGK KRAEFEISSS ASKEEILAFA KENTAKWLEG KSIVKEIYVE

GKLVNLVIK
Length:809
Mass (Da):93,384
Last modified:November 13, 2007 - v1
Checksum:i4F211E934AD03141
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000814 Genomic DNA. Translation: ABV52631.1.
RefSeqiYP_001482608.1. NC_009839.1.

Genome annotation databases

EnsemblBacteriaiABV52631; ABV52631; C8J_1032.
KEGGicju:C8J_1032.
PATRICi20055696. VBICamJej119085_1042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000814 Genomic DNA. Translation: ABV52631.1.
RefSeqiYP_001482608.1. NC_009839.1.

3D structure databases

ProteinModelPortaliA8FME4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi407148.C8J_1032.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV52631; ABV52631; C8J_1032.
KEGGicju:C8J_1032.
PATRICi20055696. VBICamJej119085_1042.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCJEJ407148:GHCS-1067-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Campylobacter jejuni strain 81116 (NCTC11828)."
    Pearson B.M., Gaskin D.J.H., Segers R.P.A.M., Wells J.M., Nuijten P.J.M., van Vliet A.H.M.
    J. Bacteriol. 189:8402-8403(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 81116 / NCTC 11828.

Entry informationi

Entry nameiSYL_CAMJ8
AccessioniPrimary (citable) accession number: A8FME4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 13, 2007
Last modified: April 1, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.