A8FLV4 (ALR_CAMJ8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	C8J_0842

Organism

Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) [Complete proteome] [HAMAP]

Taxonomic identifier

407148 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter › Campylobacter jejuni › Campylobacter jejuni subsp. jejuni

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids By similarity. HAMAP-Rule MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP-Rule MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP-Rule MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP-Rule MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	D-alanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	alanine racemase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: UniProtKB-HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Alanine racemase HAMAP-Rule MF_01201		PRO_1000138586
Sites
	Active site	33	1	Proton acceptor; specific for D-alanine By similarity
	Active site	237	1	Proton acceptor; specific for L-alanine By similarity
	Binding site	118	1	Substrate By similarity
	Binding site	283	1	Substrate; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	33	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FLV4 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 5D76BB81EDD9BF98	FASTA	328	37,275
	10 20 30 40 50 60 MSLIKIDQKA YEYNLRHIAK KIGSFQRLIC VFKDNAYGHG AKLLAPLAKN LGVSFVAVKS 70 80 90 100 110 120 EEEAQEIEEF FENILILSHR PHGNENSRFI YALNDISQVK KYKQDIKIHL KIDTGMHRNG 130 140 150 160 170 180 ICVENLEHAI DLIRSSDLKL TGMFTHFASA DEMDGSFFVQ KENFQKAKKI VKKYFSNLLF 190 200 210 220 230 240 HSHNSAALFR GKIPEDEYCR VGLVQFGYGD SNLKRVLSLY AHRLSQRILQ KGQSIGYGGI 250 260 270 280 290 300 FTAAKDMEVA TYDLGYADGL FRYNGKGELV LGNGKVMLGK MSMDSFSCEN SGEEICVFKD 310 320 ADIWADFFHT INYEILVKLN PNIQRVLV

References

[1]

"The complete genome sequence of Campylobacter jejuni strain 81116 (NCTC11828)." Pearson B.M., Gaskin D.J.H., Segers R.P.A.M., Wells J.M., Nuijten P.J.M., van Vliet A.H.M. J. Bacteriol. 189:8402-8403(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 81116 / NCTC 11828.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000814 Genomic DNA. Translation: ABV52441.1.
RefSeq	YP_001482418.1. NC_009839.1.
3D structure databases
ProteinModelPortal	A8FLV4.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	407148.C8J_0842.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV52441; ABV52441; C8J_0842.
GeneID	5617191.
KEGG	cju:C8J_0842.
PATRIC	20055294. VBICamJej119085_0850.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HOG000031446.
KO	K01775.
OMA	NIRADEM.
OrthoDB	EOG6PP9NJ.
Enzyme and pathway databases
BioCyc	CJEJ407148:GHCS-868-MONOMER.
UniPathway	UPA00042; UER00497.
Family and domain databases
Gene3D	2.40.37.10. 1 hit. 3.20.20.10. 1 hit.
HAMAP	MF_01201. Ala_racemase.
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. IPR029066. PLP-binding_barrel. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. SSF50621. 1 hit. SSF51419. SSF51419. 1 hit.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_CAMJ8

Accession

Primary (citable) accession number: A8FLV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 13, 2007
Last modified:	June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program