A8FLU4 (AROA_CAMJ8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	C8J_0832

Organism

Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) [Complete proteome] [HAMAP]

Taxonomic identifier

407148 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.
Sequence caution	The sequence ABV52431.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000315382

Sequences

	Sequence		Length	Mass (Da)
	A8FLU4 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: 8660EA7D190A111E	FASTA	428	47,204
	10 20 30 40 50 60 MKIYKLQTPV NAILENIAAD KSISHRFAIF SLLTQEENKA QNYLLAQDTL NTLEIIKNLG 70 80 90 100 110 120 AKIEQKDSCV KIIPPKEILS PNCILDCGNS GTAMRLMIGF LAGISGFFVL SGDKYLNNRP 130 140 150 160 170 180 MRRISKPLTQ IGARIYGRNE ANLAPLCIEG QNLKAFNYKS EISSAQVKTA MILSAFRANN 190 200 210 220 230 240 VCAFSEISLS RNHSENMLKA MKAPIRVSND GLSLEISPLK KPLKAQNIII PNDPSSAFYF 250 260 270 280 290 300 ALAAIILPKS QIILKNILLN PTRIEAYKIL QKMGAKLEMT ITQNDFETIG EIRVESSKLN 310 320 330 340 350 360 GIEVKDNIAW LIDEAPALAI AFALAKGKSS LINAKELRVK ESDRIAVMVE NLKLCGVEAR 370 380 390 400 410 420 ELDDGFEIEG GCELKSSKIK SYGDHRIAMS FAILGLLCGI EIDDSDCIKT SFPNFIEILS NLGARIDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The aroA gene of Campylobacter jejuni." Woesten M.M.S.M., Dubbink V.H.J., van der Zeijst B.A.M. Gene 181:109-112(1996) [PubMed: 8973316] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Campylobacter jejuni strain 81116 (NCTC11828)." Pearson B.M., Gaskin D.J.H., Segers R.P.A.M., Wells J.M., Nuijten P.J.M., van Vliet A.H.M. J. Bacteriol. 189:8402-8403(2007) [PubMed: 17873037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 81116 / NCTC 11828.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89371 Genomic DNA. Translation: CAA61554.1. CP000814 Genomic DNA. Translation: ABV52431.1. Different initiation.
PIR	JC5338.
RefSeq	YP_001482408.1. NC_009839.1.
3D structure databases
ProteinModelPortal	A8FLU4.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FLU4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5617076.
GenomeReviews	Gene locus C8J_0832 in contig CP000814_GR.
KEGG	cju:C8J_0832.
PATRIC	20055274. VBICamJej119085_0840.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0128.
HOGENOM	HBG646626.
OMA	LECANIS.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	CJEJ407148:C8J_0832-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_CAMJ8

Accession

Primary (citable) accession number: A8FLU4
Secondary accession number(s): P52312, Q0PA03, Q9PP36

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program