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Protein

Tetraacyldisaccharide 4'-kinase

Gene

lpxK

Organism
Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).UniRule annotation

Catalytic activityi

ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi63 – 70ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00359; UER00482

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraacyldisaccharide 4'-kinaseUniRule annotation (EC:2.7.1.130UniRule annotation)
Alternative name(s):
Lipid A 4'-kinaseUniRule annotation
Gene namesi
Name:lpxKUniRule annotation
Ordered Locus Names:C8J_0762
OrganismiCampylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828)
Taxonomic identifieri407148 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000722751 – 308Tetraacyldisaccharide 4'-kinaseAdd BLAST308

Structurei

3D structure databases

SMRiA8FLM4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxK family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000103105
KOiK00912
OMAiDDGFRFN

Family and domain databases

HAMAPiMF_00409 LpxK, 1 hit
InterProiView protein in InterPro
IPR003758 Tetraacyldisaccharide_4-kinase
PANTHERiPTHR42724 PTHR42724, 2 hits
PfamiView protein in Pfam
PF02606 LpxK, 2 hits

Sequencei

Sequence statusi: Complete.

A8FLM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEEKNYELW LDNYFFKPNF WQKCLAFILL PLSVFYAFFA ILNTFFRKKI
60 70 80 90 100
VFKKPVISVG NLSFGGNGKT PLCKAIAREF DGVFIVLRGY KRKSKGLFVV
110 120 130 140 150
KNQNEILCTL TQSGDEAMEY AFEENIKGVI VSEDRVKGIE KAFELGAKIV
160 170 180 190 200
VLDDAFSKFH IKKFDILLES KIKPYFNFTL PSGAYRLPKF YEKRADFIAL
210 220 230 240 250
EGRDFVRYSF VKENPKAVLV TAIAKPFRLY EHFIKARACY FFKDHYEFKK
260 270 280 290 300
EELENLLKKH NCDTLMLTFK DFVKVKDFGF KCQIIELNIE LKDSLREKIK

TYIKEFEQ
Length:308
Mass (Da):36,235
Last modified:November 13, 2007 - v1
Checksum:iA1237FEAC96229D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000814 Genomic DNA Translation: ABV52361.1
RefSeqiWP_002865989.1, NC_009839.1

Genome annotation databases

EnsemblBacteriaiABV52361; ABV52361; C8J_0762
KEGGicju:C8J_0762

Similar proteinsi

Entry informationi

Entry nameiLPXK_CAMJ8
AccessioniPrimary (citable) accession number: A8FLM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 13, 2007
Last modified: April 25, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health