A8FLM3 (NADE_CAMJ8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	C8J_0761

Organism

Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) [Complete proteome] [HAMAP]

Taxonomic identifier

407148 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077540
Regions
	Nucleotide binding	29 – 36	8	ATP By similarity
Sites
	Active site	31	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FLM3 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 5C2C8395262B624A	FASTA	246	27,493
	10 20 30 40 50 60 MDWQKITEKM CDFIQEKVKN SQSQGVVLGL SGGIDSALVA TLCKRALKEN VFALLMPTQI 70 80 90 100 110 120 SNKANLEDAL RLCADLNLEY KIIEIQSILD AFIKQSENTT LVSLGNFAAR IRMSLLYDYS 130 140 150 160 170 180 ALKNSLVIGT SNKSELLLGY GTIYGDLACA FNPIGSLYKS EIYTLAKYLN LHENFIKKAP 190 200 210 220 230 240 SADLWENQSD EADLGFSYAK IDEGLKALET NDEKLLRTLD PSLIAMLKNR MQKNTFKGKM PEILEI

References

[1]

"The complete genome sequence of Campylobacter jejuni strain 81116 (NCTC11828)." Pearson B.M., Gaskin D.J.H., Segers R.P.A.M., Wells J.M., Nuijten P.J.M., van Vliet A.H.M. J. Bacteriol. 189:8402-8403(2007) [PubMed: 17873037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 81116 / NCTC 11828.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000814 Genomic DNA. Translation: ABV52360.1.
RefSeq	YP_001482337.1. NC_009839.1.
3D structure databases
ProteinModelPortal	A8FLM3.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FLM3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5618142.
GenomeReviews	Gene locus C8J_0761 in contig CP000814_GR.
KEGG	cju:C8J_0761.
PATRIC	20055128. VBICamJej119085_0769.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	YDISARD.
ProtClustDB	PRK13980.
Enzyme and pathway databases
BioCyc	CJEJ407148:C8J_0761-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_CAMJ8

Accession

Primary (citable) accession number: A8FLM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program