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A8FLJ3

- NAPA_CAMJ8

UniProt

A8FLJ3 - NAPA_CAMJ8

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Protein
Periplasmic nitrate reductase
Gene
napA, C8J_0731
Organism
Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi42 – 421Iron-sulfur (4Fe-4S) By similarity
Metal bindingi45 – 451Iron-sulfur (4Fe-4S) By similarity
Metal bindingi49 – 491Iron-sulfur (4Fe-4S) By similarity
Metal bindingi77 – 771Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciCJEJ407148:GHCS-755-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:C8J_0731
OrganismiCampylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828)
Taxonomic identifieri407148 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000002013: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Tat-type signal Reviewed prediction
Add
BLAST
Chaini31 – 924894Periplasmic nitrate reductaseUniRule annotation
PRO_1000073641Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi407148.C8J_0731.

Structurei

3D structure databases

ProteinModelPortaliA8FLJ3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 91574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiFNQHQRG.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A8FLJ3-1 [UniParc]FASTAAdd to Basket

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MNRRDFIKNT AIASAASVAG LSVPSSMLGA QEEDWKWDKA VCRFCGTGCG    50
IMIARKDGKI VATKGDPAAP VNRGLNCIKG YFNAKIMYGE DRLVMPLLRM 100
NEKGEFDKKG KFQQVSWQRA FDEMEKQFKK AYNELGVTGI GIFGSGQYTI 150
QEGYAALKLA KAGFRTNNID PNARHCMASA VVGFMQTFGV DEPSGCYDDI 200
ELTDTIITWG ANMAEMHPIL WSRVSDRKLS NLDKVKVVNL STFSNRTSNI 250
ADIEIIFKPN TDLAIWNYIA REIVYNHPEA MDMKFIKDHC VFATGYADIG 300
YGMRNNPNHP KFKESEKDTV EKENVITLDD EEATSLSYLG VKAGDKFEMK 350
HQGVADKNWE ISFEEFKKGL APYTLEYTAK VAKGDDNESL EDFKKKLQEL 400
ANLYIEKNRK VVSFWTMGFN QHTRGSWVNE QAYMVHFLLG KQAKPGSGAF 450
SLTGQPSACG TAREVGTFSH RLPADMVVAN PKHREISEKI WKVPAKTINP 500
KPGSPYLNIM RDLEDGKIKF AWVQVNNPWQ NTANANHWIA AAREMDNFIV 550
VSDCYPGISA KVADLILPSA MIYEKWGAYG NAERRTQHWK QQVLPVGAAM 600
SDTWQILEFA KRFKLKEVWK EQKVDNKLTL PSVLEEAKAM GYSEDDTLFD 650
VLFANKEAKS FNPNDAIAKG FDNTDVKGDE RKIQGSDGKE FAGYGFFVQK 700
YLWEEYRKFG LGHGHDLADF DTYHKVRGLR WPVVNGKETQ WRFNTKFDYY 750
AKKAAPNSDF AFYGDFNKML TNGDLIAPKD EKEHSIKNKA KIFFRPFMKA 800
PERPSKEYPF WLATGRVLEH WHSGTMTMRV PELYRAVPEA LCYMSEKDGE 850
KLGLNQGDLV WVESRRGKVK ARVDMRGRNK PPVGLVYVPW FDENVYINKV 900
TLDATCPLSK QTDFKKCAVK IYKA 924
Length:924
Mass (Da):104,900
Last modified:November 13, 2007 - v1
Checksum:i7F5EA4A1C9176630
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000814 Genomic DNA. Translation: ABV52330.1.
RefSeqiWP_002866908.1. NC_009839.1.
YP_001482307.1. NC_009839.1.

Genome annotation databases

EnsemblBacteriaiABV52330; ABV52330; C8J_0731.
GeneIDi5617060.
KEGGicju:C8J_0731.
PATRICi20055068. VBICamJej119085_0739.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000814 Genomic DNA. Translation: ABV52330.1 .
RefSeqi WP_002866908.1. NC_009839.1.
YP_001482307.1. NC_009839.1.

3D structure databases

ProteinModelPortali A8FLJ3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 407148.C8J_0731.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV52330 ; ABV52330 ; C8J_0731 .
GeneIDi 5617060.
KEGGi cju:C8J_0731.
PATRICi 20055068. VBICamJej119085_0739.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi FNQHQRG.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci CJEJ407148:GHCS-755-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Campylobacter jejuni strain 81116 (NCTC11828)."
    Pearson B.M., Gaskin D.J.H., Segers R.P.A.M., Wells J.M., Nuijten P.J.M., van Vliet A.H.M.
    J. Bacteriol. 189:8402-8403(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 81116 / NCTC 11828.

Entry informationi

Entry nameiNAPA_CAMJ8
AccessioniPrimary (citable) accession number: A8FLJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 13, 2007
Last modified: September 3, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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