Skip Header

Contribute Send feedback
Read comments (?) or add your own

A8FLJ3 (NAPA_CAMJ8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase
EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:C8J_0731
OrganismCampylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) [Complete proteome] [HAMAP]
Taxonomic identifier407148 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter ›

Protein attributes

Sequence length924 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity.

Binds 1 molybdenum ion per subunit By similarity.

Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.

Subunit structure

Interacts with NapB By similarity.

Subcellular location

Periplasm By similarity.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030Tat-type signal Potential
Chain31 – 924894Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000073641

Sites

Metal binding421Iron-sulfur (4Fe-4S) By similarity
Metal binding451Iron-sulfur (4Fe-4S) By similarity
Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding771Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A8FLJ3 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: 7F5EA4A1C9176630

FASTA924104,900
        10         20         30         40         50         60 
MNRRDFIKNT AIASAASVAG LSVPSSMLGA QEEDWKWDKA VCRFCGTGCG IMIARKDGKI 

        70         80         90        100        110        120 
VATKGDPAAP VNRGLNCIKG YFNAKIMYGE DRLVMPLLRM NEKGEFDKKG KFQQVSWQRA 

       130        140        150        160        170        180 
FDEMEKQFKK AYNELGVTGI GIFGSGQYTI QEGYAALKLA KAGFRTNNID PNARHCMASA 

       190        200        210        220        230        240 
VVGFMQTFGV DEPSGCYDDI ELTDTIITWG ANMAEMHPIL WSRVSDRKLS NLDKVKVVNL 

       250        260        270        280        290        300 
STFSNRTSNI ADIEIIFKPN TDLAIWNYIA REIVYNHPEA MDMKFIKDHC VFATGYADIG 

       310        320        330        340        350        360 
YGMRNNPNHP KFKESEKDTV EKENVITLDD EEATSLSYLG VKAGDKFEMK HQGVADKNWE 

       370        380        390        400        410        420 
ISFEEFKKGL APYTLEYTAK VAKGDDNESL EDFKKKLQEL ANLYIEKNRK VVSFWTMGFN 

       430        440        450        460        470        480 
QHTRGSWVNE QAYMVHFLLG KQAKPGSGAF SLTGQPSACG TAREVGTFSH RLPADMVVAN 

       490        500        510        520        530        540 
PKHREISEKI WKVPAKTINP KPGSPYLNIM RDLEDGKIKF AWVQVNNPWQ NTANANHWIA 

       550        560        570        580        590        600 
AAREMDNFIV VSDCYPGISA KVADLILPSA MIYEKWGAYG NAERRTQHWK QQVLPVGAAM 

       610        620        630        640        650        660 
SDTWQILEFA KRFKLKEVWK EQKVDNKLTL PSVLEEAKAM GYSEDDTLFD VLFANKEAKS 

       670        680        690        700        710        720 
FNPNDAIAKG FDNTDVKGDE RKIQGSDGKE FAGYGFFVQK YLWEEYRKFG LGHGHDLADF 

       730        740        750        760        770        780 
DTYHKVRGLR WPVVNGKETQ WRFNTKFDYY AKKAAPNSDF AFYGDFNKML TNGDLIAPKD 

       790        800        810        820        830        840 
EKEHSIKNKA KIFFRPFMKA PERPSKEYPF WLATGRVLEH WHSGTMTMRV PELYRAVPEA 

       850        860        870        880        890        900 
LCYMSEKDGE KLGLNQGDLV WVESRRGKVK ARVDMRGRNK PPVGLVYVPW FDENVYINKV 

       910        920 
TLDATCPLSK QTDFKKCAVK IYKA

« Hide

References

[1]"The complete genome sequence of Campylobacter jejuni strain 81116 (NCTC11828)."
Pearson B.M., Gaskin D.J.H., Segers R.P.A.M., Wells J.M., Nuijten P.J.M., van Vliet A.H.M.
J. Bacteriol. 189:8402-8403(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 81116 / NCTC 11828.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000814 Genomic DNA. Translation: ABV52330.1.
RefSeqYP_001482307.1. NC_009839.1.

3D structure databases

ProteinModelPortalA8FLJ3.
ModBaseSearch...

Protein-protein interaction databases

STRING407148.C8J_0731.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV52330; ABV52330; C8J_0731.
GeneID5617060.
KEGGcju:C8J_0731.
PATRIC20055068. VBICamJej119085_0739.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAHWIKAAR.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycCJEJ407148:GHCS-775-MONOMER.

Family and domain databases

Gene3D2.40.40.20. 2 hits.
HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_Fe4S4_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00490. MOLYBDOPTERIN_PROK_2. False negative.
PS00932. MOLYBDOPTERIN_PROK_3. False negative.
PS51318. TAT. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_CAMJ8
AccessionPrimary (citable) accession number: A8FLJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 13, 2007
Last modified: May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents