Reviewed, UniProtKB/Swiss-Prot A8FKL9 (MURD_CAMJ8)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	C8J_0407

Organism

Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) [Complete proteome] [HAMAP]

Taxonomic identifier

407148 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000072681
Regions
	Nucleotide binding	97 – 103	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A8FKL9-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: F3B39230BAC84DE8	FASTA	402	45,423
	10 20 30 40 50 60 MKISLFGYGK TTRAIAENLV DKFGPFDIYD DHFTETKKDT LGNLLLNPND FDDNLSDIEI 70 80 90 100 110 120 PSPGFPPKHK LIQKAKNLQS EYDFFYDIMP KSVWISGTNG KTTTTQMATH LLSHIGAVIG 130 140 150 160 170 180 GNVGTPLAEL DPYAKLWILE TSSFTLHYTH KAKPEIYALL PISPDHLSWH GSFDNYVQDK 190 200 210 220 230 240 LSVLKRMNEC DVVILPKIYA NTPTKAHKIS YKDEKDLAVK FGIDTEKISF KSPFLLDAIM 250 260 270 280 290 300 ALAIEKILLD TLSYELLNSF VMEKNKLEEL KDSQNRLWVN DTKATNESAV MAALNRYKDK 310 320 330 340 350 360 KIHLIIGGDD KGVDLSNLFD FMKNFNIELY AIGISTEKML DYAKKANLKA YKCEVLSKAV 370 380 390 400 NEISNHLRVN EVALLSPACA SLDQFNSYVE RGKVFKECVN KI

References

[1]	"The complete genome sequence of Campylobacter jejuni strain 81116 (NCTC11828)." Pearson B.M., Gaskin D.J.H., Segers R.P.A.M., Wells J.M., Nuijten P.J.M., van Vliet A.H.M. J. Bacteriol. 189:8402-8403(2007) [PubMed: 17873037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000814 Genomic DNA. Translation: ABV52006.1.
RefSeq	YP_001481983.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8FKL9.
Genome annotation databases
GeneID	5617648.
GenomeReviews	Gene locus C8J_0407 in contig CP000814_GR.
KEGG	cju:C8J_0407.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LWVNDTK.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_CAMJ8

Accession

Primary (citable) accession number: A8FKL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)