Reviewed, UniProtKB/Swiss-Prot A8FKB5 (SERC_CAMJ8)

Names and origin

Protein names

Recommended name:
    Phosphoserine aminotransferase
    EC=2.6.1.52
Alternative name(s):
    Phosphohydroxythreonine aminotransferase
      Short name=PSAT

Gene names

Name:	serC
Ordered Locus Names:	C8J_0303

Organism

Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) [Complete proteome] [HAMAP]

Taxonomic identifier

407148 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine By similarity.
Catalytic activity	O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate. HAMAP MF_00160 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate. HAMAP MF_00160
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glyceric acid: step 2/3. HAMAP MF_00160 Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5. HAMAP MF_00160
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Pyridoxine biosynthesis Serine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	O-phospho-L-serine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Phosphoserine aminotransferase HAMAP MF_00160		PRO_1000071542
Regions
	Region	75 – 76	2	Pyridoxal phosphate binding By similarity
	Region	233 – 234	2	Pyridoxal phosphate binding By similarity
Sites
	Binding site	41	1	L-glutamate By similarity
	Binding site	100	1	Pyridoxal phosphate By similarity
	Binding site	148	1	Pyridoxal phosphate By similarity
	Binding site	167	1	Pyridoxal phosphate By similarity
	Binding site	190	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	191	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FKB5-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 39BD4191ACCA9BC6	FASTA	358	40,441
	10 20 30 40 50 60 MRKINFSAGP STLPLEILEQ AQKELCDYQG RGYSIMEISH RTKVFEEVHF GAQEKAKKLY 70 80 90 100 110 120 GLNDDYEVLF LQGGASLQFA MIPMNLSLNG VCEYANTGVW TKKAIKEAQI LGVNVKTVAS 130 140 150 160 170 180 SEESNFNHIP RVEFSDNADY AYICSNNTIY GTQYQNYPKT KAPLIVDASS DFFSRKVDFS 190 200 210 220 230 240 NIALFYGGVQ KNAGISGLSC IFIRKDMLER SKNKQIPSML NYLTHAENQS LFNTPPTFAI 250 260 270 280 290 300 YMFNLEMDWL LNQGELDKVH EKNSQKAAML YECIDLSNGF YKGHADKKDR SLMNVSFNIA 310 320 330 340 350 KNKDLEPLFV KEAEEAGMIG LKGHRILGGI RASIYNALNL DQVKTLCEFM KEFQGKYA

References

[1]	"The complete genome sequence of Campylobacter jejuni strain 81116 (NCTC11828)." Pearson B.M., Gaskin D.J.H., Segers R.P.A.M., Wells J.M., Nuijten P.J.M., van Vliet A.H.M. J. Bacteriol. 189:8402-8403(2007) [PubMed: 17873037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000814 Genomic DNA. Translation: ABV51902.1.
RefSeq	YP_001481879.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5618480.
GenomeReviews	Gene locus C8J_0303 in contig CP000814_GR.
KEGG	cju:C8J_0303.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8FKB5. SMYNTPP.
Family and domain databases
HAMAP	MF_00160. [Tree]
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR003248. Pser_amintransf. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
ProDom	PD001544. Pser_amintransf. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01364. serC_1. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERC_CAMJ8

Accession

Primary (citable) accession number: A8FKB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	November 13, 2007
Last modified:	June 16, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)