A8FK97 (BIOD_CAMJ8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	C8J_0285

Organism

Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) [Complete proteome] [HAMAP]

Taxonomic identifier

407148 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_1000072046
Regions
	Nucleotide binding	93 – 96	4	ATP By similarity
Sites
	Metal binding	11	1	Magnesium 1 By similarity
	Metal binding	15	1	Magnesium 2 By similarity
	Metal binding	40	1	Magnesium 2 By similarity
	Metal binding	93	1	Magnesium 2 By similarity
	Binding site	40	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FK97 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: D20A0E57E9EE36B8	FASTA	201	22,983
	10 20 30 40 50 60 MQIYVSGIHT DVGKTHFSAA FCANFNYDYF KLIQAGTPTD SEFIAKFSPK TKIFKEGIFL 70 80 90 100 110 120 QTPASPHLGK IKEKLDYKAL DIILPKSKNL LIELAGGLFS PMDENYTMID FVNIFKHPTI 130 140 150 160 170 180 LVAKYYLGSI NHILLSIEAL KQRNINLLAL VMMGKKDILQ DDFIKNYAKI PIINLDFFDE 190 200 NSILNKDFKE QMQEILQLKI P

References

[1]

"The complete genome sequence of Campylobacter jejuni strain 81116 (NCTC11828)." Pearson B.M., Gaskin D.J.H., Segers R.P.A.M., Wells J.M., Nuijten P.J.M., van Vliet A.H.M. J. Bacteriol. 189:8402-8403(2007) [PubMed: 17873037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 81116 / NCTC 11828.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000814 Genomic DNA. Translation: ABV51884.1.
RefSeq	YP_001481861.1. NC_009839.1.
3D structure databases
ProteinModelPortal	A8FK97.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FK97.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5616988.
GenomeReviews	Gene locus C8J_0285 in contig CP000814_GR.
KEGG	cju:C8J_0285.
PATRIC	20054140. VBICamJej119085_0293.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0132.
HOGENOM	HBG650065.
OMA	WKPIQSG.
ProtClustDB	CLSK878731.
Enzyme and pathway databases
BioCyc	CJEJ407148:C8J_0285-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
PIRSF	PIRSF006755. DTB_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CAMJ8

Accession

Primary (citable) accession number: A8FK97

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program