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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Bacillus pumilus (strain SAFR-032)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45Substrate; via amide nitrogenUniRule annotation1
Binding sitei121ATPUniRule annotation1
Binding sitei168ATPUniRule annotation1
Binding sitei195Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBPUM315750:G1G9U-3532-MONOMER
UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:BPUM_3484
OrganismiBacillus pumilus (strain SAFR-032)
Taxonomic identifieri315750 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001355 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000587581 – 271Hydroxyethylthiazole kinaseAdd BLAST271

Proteomic databases

PRIDEiA8FIR7

Interactioni

Protein-protein interaction databases

STRINGi315750.BPUM_3484

Structurei

3D structure databases

ProteinModelPortaliA8FIR7
SMRiA8FIR7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4U Bacteria
COG2145 LUCA
HOGENOMiHOG000114352
KOiK00878
OMAiKRPLVHN
OrthoDBiPOG091H004S

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00694 thiM, 1 hit

Sequencei

Sequence statusi: Complete.

A8FIR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTSIASHLL EKVRAENPLV HNITNQVVTN FTANGLLALG ASPVMANAKE
60 70 80 90 100
EVAEMAQLAD ALVLNIGTLT KETVESMILA GQSANKKGIP VLLDPVGVGA
110 120 130 140 150
TTFRLKAAKQ LLEQVNITVV RGNAAEIAHL LEVDGWESKG VDAKAANGDV
160 170 180 190 200
SALVKQAAKT LQTVVVITGE VDVVSDGEDV LSIHNGHEWL TKVTGTGCLL
210 220 230 240 250
TSVIGAFCAA GERPLHASAA ALLFYGVAAE KAAQYTQNKG PGTFQMELLN
260 270
ALSHTTGNDV LTLGKIGRNV T
Length:271
Mass (Da):28,236
Last modified:November 13, 2007 - v1
Checksum:i8AF7EBBAD1C4F9FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000813 Genomic DNA Translation: ABV64134.1
RefSeqiWP_012011689.1, NC_009848.4

Genome annotation databases

EnsemblBacteriaiABV64134; ABV64134; BPUM_3484
KEGGibpu:BPUM_3484

Similar proteinsi

Entry informationi

Entry nameiTHIM_BACP2
AccessioniPrimary (citable) accession number: A8FIR7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: May 23, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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