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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Bacillus pumilus (strain SAFR-032)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451Substrate; via amide nitrogenUniRule annotation
Binding sitei121 – 1211ATPUniRule annotation
Binding sitei168 – 1681ATPUniRule annotation
Binding sitei195 – 1951Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBPUM315750:GH6N-3566-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:BPUM_3484
OrganismiBacillus pumilus (strain SAFR-032)
Taxonomic identifieri315750 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001355 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Hydroxyethylthiazole kinasePRO_1000058758Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi315750.BPUM_3484.

Structurei

3D structure databases

ProteinModelPortaliA8FIR7.
SMRiA8FIR7. Positions 1-268.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

A8FIR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTSIASHLL EKVRAENPLV HNITNQVVTN FTANGLLALG ASPVMANAKE
60 70 80 90 100
EVAEMAQLAD ALVLNIGTLT KETVESMILA GQSANKKGIP VLLDPVGVGA
110 120 130 140 150
TTFRLKAAKQ LLEQVNITVV RGNAAEIAHL LEVDGWESKG VDAKAANGDV
160 170 180 190 200
SALVKQAAKT LQTVVVITGE VDVVSDGEDV LSIHNGHEWL TKVTGTGCLL
210 220 230 240 250
TSVIGAFCAA GERPLHASAA ALLFYGVAAE KAAQYTQNKG PGTFQMELLN
260 270
ALSHTTGNDV LTLGKIGRNV T
Length:271
Mass (Da):28,236
Last modified:November 13, 2007 - v1
Checksum:i8AF7EBBAD1C4F9FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000813 Genomic DNA. Translation: ABV64134.1.
RefSeqiWP_012011689.1. NC_009848.1.
YP_001488694.1. NC_009848.1.

Genome annotation databases

EnsemblBacteriaiABV64134; ABV64134; BPUM_3484.
GeneIDi5622779.
KEGGibpu:BPUM_3484.
PATRICi18970938. VBIBacPum16546_3538.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000813 Genomic DNA. Translation: ABV64134.1.
RefSeqiWP_012011689.1. NC_009848.1.
YP_001488694.1. NC_009848.1.

3D structure databases

ProteinModelPortaliA8FIR7.
SMRiA8FIR7. Positions 1-268.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315750.BPUM_3484.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV64134; ABV64134; BPUM_3484.
GeneIDi5622779.
KEGGibpu:BPUM_3484.
PATRICi18970938. VBIBacPum16546_3538.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.
OrthoDBiEOG628F8M.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciBPUM315750:GH6N-3566-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SAFR-032.

Entry informationi

Entry nameiTHIM_BACP2
AccessioniPrimary (citable) accession number: A8FIR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: April 29, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.