A8FIR7 (THIM_BACP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyethylthiazole kinase
EC=2.7.1.50

Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase

Gene names

Name:	thiM
Ordered Locus Names:	BPUM_3484

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus pumilus

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW thiamine diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Hydroxyethylthiazole kinase HAMAP-Rule MF_00228		PRO_1000058758
Sites
	Binding site	45	1	Substrate; via amide nitrogen By similarity
	Binding site	121	1	ATP By similarity
	Binding site	168	1	ATP By similarity
	Binding site	195	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FIR7 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 8AF7EBBAD1C4F9FB	FASTA	271	28,236
	10 20 30 40 50 60 MKTSIASHLL EKVRAENPLV HNITNQVVTN FTANGLLALG ASPVMANAKE EVAEMAQLAD 70 80 90 100 110 120 ALVLNIGTLT KETVESMILA GQSANKKGIP VLLDPVGVGA TTFRLKAAKQ LLEQVNITVV 130 140 150 160 170 180 RGNAAEIAHL LEVDGWESKG VDAKAANGDV SALVKQAAKT LQTVVVITGE VDVVSDGEDV 190 200 210 220 230 240 LSIHNGHEWL TKVTGTGCLL TSVIGAFCAA GERPLHASAA ALLFYGVAAE KAAQYTQNKG 250 260 270 PGTFQMELLN ALSHTTGNDV LTLGKIGRNV T

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAFR-032.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000813 Genomic DNA. Translation: ABV64134.1.
RefSeq	YP_001488694.1. NC_009848.1.
3D structure databases
ProteinModelPortal	A8FIR7.
SMR	A8FIR7. Positions 1-268.
ModBase	Search...
Protein-protein interaction databases
STRING	315750.BPUM_3484.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV64134; ABV64134; BPUM_3484.
GeneID	5622779.
KEGG	bpu:BPUM_3484.
PATRIC	18970938. VBIBacPum16546_3538.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2145.
HOGENOM	HOG000114352.
KO	K00878.
OMA	SPVMAHA.
ProtClustDB	PRK09355.
Enzyme and pathway databases
BioCyc	BPUM315750:GH6N-3570-MONOMER.
UniPathway	UPA00060; UER00139.
Family and domain databases
HAMAP	MF_00228. Thz_kinase.
InterPro	IPR000417. Hyethyz_kinase. [Graphical view]
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. thiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_BACP2

Accession

Primary (citable) accession number: A8FIR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program