A8FIM7 (UNG_BACP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uracil-DNA glycosylase
Short name=UDG
EC=3.2.2.27

Gene names

Name:	ung
Ordered Locus Names:	BPUM_3444

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus pumilus

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine By similarity. HAMAP-Rule MF_00148
Catalytic activity	Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil. HAMAP-Rule MF_00148
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00148.
Sequence similarities	Belongs to the uracil-DNA glycosylase family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	base-excision repair Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	uracil DNA N-glycosylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Uracil-DNA glycosylase HAMAP-Rule MF_00148		PRO_1000058124
Sites
	Active site	65	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FIM7 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 1630DF38F1789739	FASTA	226	26,174
	10 20 30 40 50 60 MKPFLNDSWW AVMKSEFEQP YYQELREWMK EEYRTQTVFP KPDDVYRALH LTSYEEVKVV 70 80 90 100 110 120 ILGQDPYHGP GQAHGLSFSV QPGVKHPPSL RNIFQELKDD LGCPVPNHGS LVSWAEQGVL 130 140 150 160 170 180 LLNTVLTVRK GEANSHKGKG WERVTDRVID ALNERDQPVV FVLWGRHAQN KKERIDQNKH 190 200 210 220 YIIESPHPSP FSARNGFFGS RPFSKVNAYL KQMGTEEINW CIQDIE

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAFR-032.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000813 Genomic DNA. Translation: ABV64094.1.
RefSeq	YP_001488654.1. NC_009848.1.
3D structure databases
ProteinModelPortal	A8FIM7.
SMR	A8FIM7. Positions 1-223.
ModBase	Search...
Protein-protein interaction databases
STRING	315750.BPUM_3444.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV64094; ABV64094; BPUM_3444.
GeneID	5622739.
KEGG	bpu:BPUM_3444.
PATRIC	18970858. VBIBacPum16546_3498.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0692.
HOGENOM	HOG000229528.
KO	K03648.
OMA	KEPINWK.
ProtClustDB	PRK05254.
Enzyme and pathway databases
BioCyc	BPUM315750:GH6N-3526-MONOMER.
Family and domain databases
Gene3D	3.40.470.10. 1 hit.
HAMAP	MF_00148. UDG.
InterPro	IPR018085. Ura-DNA_Glyclase_AS. IPR002043. Ura_DNA_glycsylse. IPR005122. Uracil-DNA_glycosylase-like. [Graphical view]
PANTHER	PTHR11264. PTHR11264. 1 hit.
Pfam	PF03167. UDG. 1 hit. [Graphical view]
SMART	SM00986. UDG. 1 hit. [Graphical view]
SUPFAM	SSF52141. UDNA_glycsylseSF. 1 hit.
TIGRFAMs	TIGR00628. ung. 1 hit.
PROSITE	PS00130. U_DNA_GLYCOSYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UNG_BACP2

Accession

Primary (citable) accession number: A8FIM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	May 29, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program