Reviewed, UniProtKB/Swiss-Prot A8FIG8 (SYR_BACP2)

Names and origin

Protein names

Recommended name:
    Arginyl-tRNA synthetase
    EC=6.1.1.19
Alternative name(s):
    Arginine--tRNA ligase
      Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	BPUM_3383

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00123
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Arginyl-tRNA synthetase HAMAP MF_00123		PRO_1000057807
Regions
	Motif	132 – 142	11	"HIGH" region HAMAP MF_00123

Sequences

	Sequence		Length	Mass (Da)
	A8FIG8-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 4CB8A859ABC92D67	FASTA	557	62,734
	10 20 30 40 50 60 MNIAEQVKDA LKEEIIAAVV KAGLADESQV PDVLLEVPKD KTHGDYSTNM AMQLARIAKK 70 80 90 100 110 120 APRQIAEDIV KAFDKGKASI EKMDIAGPGF INFYMNNQYL TKLIPAVLEA KEAYGETNTG 130 140 150 160 170 180 GGQKVQVEFV SANPTGDLHL GHARGAAVGD SLCNILDKAG FDVSREYYIN DAGNQINNLA 190 200 210 220 230 240 LSVEVRYFEA LGLEKEMPED GYRGEDIKGI GQKLADEFGD RFVHESEEER MKFFREYGLK 250 260 270 280 290 300 YELEKLRVDL ENFRVPFDVW YSETSLYENG KIEPALETLR EKGYVFEEDG ATWLRSTDFG 310 320 330 340 350 360 DDKDRVLIKK DGSFTYLLPD IAYHKDKLDR GFDQLINIWG ADHHGYIPRM KAAIQALGYP 370 380 390 400 410 420 AGKLEVEIIQ LVHLYKNGEK MKMSKRTGKA VTMRDLIEEV GLDATRYFFA MRSAATHMDF 430 440 450 460 470 480 DLDLAISTSN ENPVYYAQYA HARICSMLRQ GEEKGYEPNL EKADFSHIQS EKEYDLLKII 490 500 510 520 530 540 GGFPEVVAEA AEKRIPHRVT NYIYDLASAL HSFYNAEKVI DVENETKTTA RLSLMKATQI 550 TLANALKLIG VSAPEKM

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000813 Genomic DNA. Translation: ABV64035.1.
RefSeq	YP_001488595.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8FIG8.
Genome annotation databases
GeneID	5622678.
GenomeReviews	Gene locus BPUM_3383 in contig CP000813_GR.
KEGG	bpu:BPUM_3383.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ADHHGYV.
Family and domain databases
HAMAP	MF_00123. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ic. IPR015945. Arg-tRNA-synth_Ic_core. IPR005148. Arg-tRNA-synth_Ic_N. IPR008909. DALR_anticod_bd. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_BACP2

Accession

Primary (citable) accession number: A8FIG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)