A8FI71 (MSCL_BACP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Large-conductance mechanosensitive channel

Gene names

Name:	mscL
Ordered Locus Names:	BPUM_3285

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell By similarity. HAMAP MF_00115
Subunit structure	Homopentamer By similarity. HAMAP MF_00115
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_00115.
Sequence similarities	Belongs to the MscL family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ionic channel
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 131	131	Large-conductance mechanosensitive channel HAMAP MF_00115		PRO_1000057753
Regions
	Transmembrane	14 – 34	21	Helical; Potential
	Transmembrane	67 – 87	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	A8FI71 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: C1D4BD5B4A7755C5	FASTA	131	14,609
	10 20 30 40 50 60 MLKEFREFAV KGNVIDLAVG VIIGGAFGKI VTSLVNDLIM PLVGIIIGGH DFSGLSIKIG 70 80 90 100 110 120 SAQILYGSFI QTVIDFLIIS FSIFIFIRYL NKLKRKKVEE EEVVETPDQT EVLLTEIRDL 130 LKHQSQSKDV Q

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAFR-032.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000813 Genomic DNA. Translation: ABV63938.1.
RefSeq	YP_001488498.1. NC_009848.1.
3D structure databases
ProteinModelPortal	A8FI71.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FI71.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000066034; EBBACP00000064367; EBBACG00000066025.
GeneID	5622580.
GenomeReviews	Gene locus BPUM_3285 in contig CP000813_GR.
KEGG	bpu:BPUM_3285.
PATRIC	18970534. VBIBacPum16546_3336.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1970.
GeneTree	EBGT00050000002096.
HOGENOM	HBG727604.
OMA	VIDFVIV.
Enzyme and pathway databases
BioCyc	BPUM315750:BPUM_3285-MONOMER.
Family and domain databases
HAMAP	MF_00115. MscL. [Tree]
InterPro	IPR019823. Mechanosensitive_channel_CS. IPR001185. MS_channel. [Graphical view]
KO	K03282.
Pfam	PF01741. MscL. 1 hit. [Graphical view]
PRINTS	PR01264. MECHCHANNEL.
SUPFAM	SSF81330. MS_channel. 1 hit.
TIGRFAMs	TIGR00220. MscL. 1 hit.
PROSITE	PS01327. MSCL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSCL_BACP2

Accession

Primary (citable) accession number: A8FI71

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program