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A8FHT5 (A8FHT5_BACP2) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UvrABC system protein B HAMAP-Rule MF_00204
Short name=Protein UvrB HAMAP-Rule MF_00204
Alternative name(s):
Excinuclease ABC subunit B HAMAP-Rule MF_00204
Gene names
Name:uvrB HAMAP-Rule MF_00204
Ordered Locus Names:BPUM_3148
OrganismBacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP] EMBL ABV63802.1
Taxonomic identifier315750 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length661 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204 SAAS SAAS024759

Subunit structure

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity. HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS024759

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS024759.

Domain

The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204

Sequence similarities

Belongs to the UvrB family. HAMAP-Rule MF_00204 RuleBase RU003587

Contains 1 UVR domain. HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS024759

Contains 1 helicase ATP-binding domain. HAMAP-Rule MF_00204 SAAS SAAS024759

Contains 1 helicase C-terminal domain. HAMAP-Rule MF_00204 SAAS SAAS024759

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain26 – 181156Helicase ATP-binding By similarity HAMAP-Rule MF_00204
Domain430 – 596167Helicase C-terminal By similarity HAMAP-Rule MF_00204
Domain625 – 66036UVR By similarity HAMAP-Rule MF_00204
Nucleotide binding39 – 468ATP By similarity HAMAP-Rule MF_00204
Motif92 – 11524Beta-hairpin By similarity HAMAP-Rule MF_00204

Sequences

Sequence LengthMass (Da)Tools
A8FHT5 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: 070FCF5EC8D38A35

FASTA66176,279
        10         20         30         40         50         60 
MSDRFELVSN YQPQGDQPKA IEKLVEGIHQ GKQHQTLLGA TGTGKTFTVS NLIKEVNKPT 

        70         80         90        100        110        120 
LVIAHNKTLA GQLYSEFKEF FPNNAVEYFV SYYDYYQPEA YVPQTDTFIE KDASINDEID 

       130        140        150        160        170        180 
KLRHSATSSL FERRDVIIIA SVSCIYGLGS PEEYRELVLS LRTEMEIERN QLLRKLVDIQ 

       190        200        210        220        230        240 
YSRNDIDFQR GTFRVRGDVV EIFPASRDEH CIRVEFFGDE IERIREVDAL TGEILGDREH 

       250        260        270        280        290        300 
VAIFPASHFV TREEKMEKAI VNIEQELEEQ LEKLRDNGKL LEAQRLEQRT RYDLEMMREM 

       310        320        330        340        350        360 
GFCSGIENYS RHLTLRPPGS TPYTLLDYFP DDFMIVVDES HVTVPQIRAM YNGDQARKQV 

       370        380        390        400        410        420 
LVDHGFRLPS ALDNRPLTFD EFEKHINHIV HVSATPGPYE LEKTPEVVEQ IIRPTGLLDP 

       430        440        450        460        470        480 
IIEVRPIEGQ IDDLIGEIHA RIEKNERVLV TTLTKKMSED LTDYLKEIGI KVNYLHSEIK 

       490        500        510        520        530        540 
TLERIEIIRD LRLGKYDVLV GINLLREGLD IPEVSLVTIL DADKEGFLRS ERSLIQTIGR 

       550        560        570        580        590        600 
AARNSEGRVI MYADKMTKSM DIAIQETKRR REQQEAYNEK YGITPQTIHK KIRDAIKATK 

       610        620        630        640        650        660 
IHEESEEYET NAAPKLSKMS KKEREKVIEK VELEMKDAAK ALDFEKAAEL RDLLLELKAE 


G

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000813 Genomic DNA. Translation: ABV63802.1.
RefSeqYP_001488362.1. NC_009848.1.

3D structure databases

ProteinModelPortalA8FHT5.
SMRA8FHT5. Positions 3-654.
ModBaseSearch...

Protein-protein interaction databases

STRING315750.BPUM_3148.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV63802; ABV63802; BPUM_3148.
GeneID5622443.
KEGGbpu:BPUM_3148.
PATRIC18970266. VBIBacPum16546_3202.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0556.
HOGENOMHOG000073580.
KOK03702.
OMACIYGLGI.
ProtClustDBPRK05298.

Enzyme and pathway databases

BioCycBPUM315750:GH6N-3234-MONOMER.

Family and domain databases

Gene3D4.10.860.10. 1 hit.
HAMAPMF_00204. UvrB.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERPTHR24029. PTHR24029. 1 hit.
PfamPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF46600. UvrB_C. 1 hit.
TIGRFAMsTIGR00631. uvrb. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA8FHT5_BACP2
AccessionPrimary (citable) accession number: A8FHT5
Entry history
Integrated into UniProtKB/TrEMBL: November 13, 2007
Last sequence update: November 13, 2007
Last modified: May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info