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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Bacillus pumilus (strain SAFR-032)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.UniRule annotation

Pathway:iglycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361SubstrateUniRule annotation
Binding sitei118 – 1181SubstrateUniRule annotation
Binding sitei151 – 1511SubstrateUniRule annotation
Binding sitei201 – 2011ATPUniRule annotation
Binding sitei323 – 3231ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi350 – 3534ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBPUM315750:GH6N-3137-MONOMER.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinaseUniRule annotation (EC:2.7.2.3UniRule annotation)
Gene namesi
Name:pgkUniRule annotation
Ordered Locus Names:BPUM_3056
OrganismiBacillus pumilus (strain SAFR-032)
Taxonomic identifieri315750 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001355 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Phosphoglycerate kinasePRO_1000057967Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei183 – 1831PhosphoserineUniRule annotation
Modified residuei299 – 2991PhosphothreonineUniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiA8FHJ3.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi315750.BPUM_3056.

Structurei

3D structure databases

ProteinModelPortaliA8FHJ3.
SMRiA8FHJ3. Positions 1-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 233Substrate bindingUniRule annotation
Regioni59 – 624Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0126.
HOGENOMiHOG000227108.
KOiK00927.
OMAiWEALDIG.
OrthoDBiEOG64N9Z0.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8FHJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKSVKDID VKGKVVFCRV DFNVPMKDGK VTDDTRIRAA LPTIEYLTGQ
60 70 80 90 100
GAKVLLASHL GRPKGQVTEE LRLTPVAKRL QELLGQEVKK ADEAYGDNVK
110 120 130 140 150
KQISDLKDGD VLVLENVRFY PGEEKNDPEL SKAFADLADV YVNDAFGAAH
160 170 180 190 200
RAHASTAGIA AYLPAVSGFL MQKELEVLGK AISNPDRPFT AIIGGAKVKD
210 220 230 240 250
KIGVIESLLD KVDNLIIGGG LAYTFVKALG HEVGKSLLEE DKVDLAKSFM
260 270 280 290 300
DRAKEKGVNF LIPTDVLVAD DFSNDANTSI VPISEIPSDL EALDIGTETR
310 320 330 340 350
EKYADVIKNS KLVVWNGPMG VFEIDAFAKG TKAIAEALAE AKDTYSVIGG
360 370 380 390
GDSAAAVEKF GLADQMSHIS TGGGASLEFM EGKELPGVTA LNDK
Length:394
Mass (Da):42,211
Last modified:November 13, 2007 - v1
Checksum:i78E7D8F14D47A1C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000813 Genomic DNA. Translation: ABV63710.1.
RefSeqiWP_012011303.1. NC_009848.1.

Genome annotation databases

EnsemblBacteriaiABV63710; ABV63710; BPUM_3056.
GeneIDi5622350.
KEGGibpu:BPUM_3056.
PATRICi18970086. VBIBacPum16546_3113.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000813 Genomic DNA. Translation: ABV63710.1.
RefSeqiWP_012011303.1. NC_009848.1.

3D structure databases

ProteinModelPortaliA8FHJ3.
SMRiA8FHJ3. Positions 1-394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315750.BPUM_3056.

Proteomic databases

PRIDEiA8FHJ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV63710; ABV63710; BPUM_3056.
GeneIDi5622350.
KEGGibpu:BPUM_3056.
PATRICi18970086. VBIBacPum16546_3113.

Phylogenomic databases

eggNOGiCOG0126.
HOGENOMiHOG000227108.
KOiK00927.
OMAiWEALDIG.
OrthoDBiEOG64N9Z0.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
BioCyciBPUM315750:GH6N-3137-MONOMER.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SAFR-032.

Entry informationi

Entry nameiPGK_BACP2
AccessioniPrimary (citable) accession number: A8FHJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: July 22, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.