A8FHJ2 (TPIS_BACP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	BPUM_3055

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147_B
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147_B Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147_B
Subunit structure	Homodimer By similarity. HAMAP MF_00147_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00147_B.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Triosephosphate isomerase HAMAP MF_00147_B		PRO_1000058108
Sites
	Active site	95	1	Electrophile By similarity
	Active site	167	1	Proton acceptor By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	11	1	Substrate By similarity
Amino acid modifications
	Modified residue	213	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FHJ2 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 59A006A9CC8841C5	FASTA	253	27,268
	10 20 30 40 50 60 MRKPIIAGNW KMNKTLGEAV SFVEEVKSSI PYPDKVEAIV CAPALFLEKL NSLSNGTDLK 70 80 90 100 110 120 IGAQNMHFEE NGAFTGEISP AALKDLGIGY SVIGHSERRE FFAETDETVN KKAHAAFKHG 130 140 150 160 170 180 IVPIICVGET LEEREAGKTN ELVADQVKKA LAGFTTQQVA ESVIAYEPIW AIGTGKSSTA 190 200 210 220 230 240 KDANDVCAHI RQTVASEYGQ EAADSLRIQY GGSVKPANIK EYMAESDIDG ALVGGASLEP 250 QSFVQLLEEG QYE

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAFR-032.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000813 Genomic DNA. Translation: ABV63709.1.
RefSeq	YP_001488269.1. NC_009848.1.
3D structure databases
ProteinModelPortal	A8FHJ2.
SMR	A8FHJ2. Positions 2-252.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FHJ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000065128; EBBACP00000063461; EBBACG00000065119.
GeneID	5622349.
GenomeReviews	Gene locus BPUM_3055 in contig CP000813_GR.
KEGG	bpu:BPUM_3055.
PATRIC	18970084. VBIBacPum16546_3112.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0149.
GeneTree	EBGT00050000002739.
HOGENOM	HBG708281.
OMA	VCVGETQ.
ProtClustDB	PRK00042.
Enzyme and pathway databases
BioCyc	BPUM315750:BPUM_3055-MONOMER.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR022896. TrioseP_Isoase_bac/euk. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01803.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. Tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_BACP2

Accession

Primary (citable) accession number: A8FHJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program