A8FH24 (KHSE_BACP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	BPUM_2886

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Homoserine kinase HAMAP MF_00384		PRO_1000060698
Regions
	Nucleotide binding	91 – 101	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A8FH24 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: E2BF2EDFE3F47103	FASTA	310	33,396
	10 20 30 40 50 60 MSKASMLFSI TVPGSTANLG PGFDSVGMAL SRYLKLSVFD HDSWLFEAES DVVSGIPPGT 70 80 90 100 110 120 DNLIYQTAKK VADHFGKTLP PVYVKVWSDI PLARGLGSSA AAIVAAIELA NQLLELNMTD 130 140 150 160 170 180 DQKLFFASEV EGHPDNAGAS LFGGLLIGLH EEDKTHAVKV KHVDIDVVVV IPFYEVLTKD 190 200 210 220 230 240 ARDVLPEDFS YKHAVSASAV SNVLVAALMT QNWPLVGEMM NKDLFHQPYR TMLVPELSKV 250 260 270 280 290 300 EHVASLKGAY GTALSGAGPT ILTLIEKGKG EGLKKQLAQN FPHCEVDLLT VPVEGVVVEH 310 DPVNQVKNVL

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAFR-032.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000813 Genomic DNA. Translation: ABV63541.1.
RefSeq	YP_001488101.1. NC_009848.1.
3D structure databases
ProteinModelPortal	A8FH24.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FH24.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000063731; EBBACP00000062064; EBBACG00000063722.
GeneID	5622180.
GenomeReviews	Gene locus BPUM_2886 in contig CP000813_GR.
KEGG	bpu:BPUM_2886.
PATRIC	18969758. VBIBacPum16546_2949.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0083.
GeneTree	EBGT00050000000874.
HOGENOM	HBG646290.
OMA	DRLHQPY.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	BPUM315750:BPUM_2886-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_BACP2

Accession

Primary (citable) accession number: A8FH24

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program