Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A8FGG0 (SYL_BACP2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BPUM_2669
OrganismBacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]
Taxonomic identifier315750 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence ABV63327.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

regulation of translational fidelity

Inferred from electronic annotation. Source: GOC

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334730

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8FGG0 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: 76385A9ECC40E579

FASTA80491,572
        10         20         30         40         50         60 
MSFQHREIEK KWQDYWLTHK TFATSDSEDK PKFYALDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LSRMKRMQGY DVLHPMGWDA FGLPAEQYAL DTGNDPAVFT EENINNFRRQ IQSLGFSYDW 

       130        140        150        160        170        180 
DREINTTDPN YYKWTQWIFL KLYEKGLAYI DEVPVNWCPA LGTVLANEEV IDGKSERGGH 

       190        200        210        220        230        240 
PVERRPMKQW MLKITAYADR LLEDLEDIDW PESIKDMQRN WIGRSEGAHV HFEIEGHDEQ 

       250        260        270        280        290        300 
FTVFTTRPDT LFGATYAVLA PEHALVEKIT TATQKEAVEA YIKEIQSKSD LERTDLAKTK 

       310        320        330        340        350        360 
TGIFTGAYAV NPLNGEKMPI WIADYVLATY GTGAIMAVPA HDERDYEFAK TFDLPIKAVV 

       370        380        390        400        410        420 
EGGDIEEEAY TGDGKHINSD FLDGLGKEEA IEKVIAWLEE HQKGEKKVTY RLRDWLFSRQ 

       430        440        450        460        470        480 
RYWGEPIPII HWEDGTSSAV SEEDLPLILP KTTEIKPSGT GESPLANIKD WVEVVDPVTG 

       490        500        510        520        530        540 
KKGRRETNTM PQWAGSCWYF LRYIDPHNSE ELASPEKLKK WLPVDVYIGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKFLYD IGVVPTKEPF MKLFNQGMIL GENNEKMSKS KGNVVNPDDI VESHGADTLR 

       610        620        630        640        650        660 
LYEMFMGPLD ASIAWSETGL DGARRFLDRV WRLFTNEDGT ISDKVTEQTG GALERSYHET 

       670        680        690        700        710        720 
VMKVTDHYEG LRFNTGISQL MVFINDAYKA DTLPKEYAEG FVKLLSPIAP HLAEELWNKL 

       730        740        750        760        770        780 
GHEGSISYEA WPQYDESKLV DDEVEIVVQL NGKVKAKLTV PADATKEQLE DLAKSDARVK 

       790        800 
EQLEGKTIRK VIAVPGKLVN IVAN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000813 Genomic DNA. Translation: ABV63327.1. Different initiation.
RefSeqYP_001487887.1. NC_009848.1.

3D structure databases

ProteinModelPortalA8FGG0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315750.BPUM_2669.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV63327; ABV63327; BPUM_2669.
GeneID5621939.
KEGGbpu:BPUM_2669.
PATRIC18969278. VBIBacPum16546_2733.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycBPUM315750:GH6N-2727-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BACP2
AccessionPrimary (citable) accession number: A8FGG0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: February 19, 2014
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries