A8FGB7 (MURC_BACP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	BPUM_2626

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm By similarity HAMAP MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000057314
Regions
	Nucleotide binding	108 – 114	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A8FGB7 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 0D34E691485CBBFC	FASTA	432	48,663
	10 20 30 40 50 60 MTVYHFVGIK GTGMSPLAQI LHDTGYTVQG SDIEKHIFTQ KALEERNIKI LPFDPDNIQP 70 80 90 100 110 120 GMTVIAGNAF PDTHPEIERA LSEGIPVIRY HKFLGEYMDS FTNVAITGAH GKTSTTGLLA 130 140 150 160 170 180 HVMQSAKPTS YLIGDGSGMG KENSEYFVFE ACEYRRHFLS YHPDYAIMTN IDFDHPDYFS 190 200 210 220 230 240 DIDDVFDAFQ NMALQVKKAI IACGDDEHLP KIQAKVPVVY YGFNEENDFQ ARNVVKNTEG 250 260 270 280 290 300 TTFDVFVRNT FYDTFYIPAY GNHNVLNSLA VIALCHYESI DVELIKGALT TFGGVKRRFN 310 320 330 340 350 360 EKVVGAQVLI DDYAHHPTEI EVTIEAARQK YPDREIVAVF QPHTFTRTQQ FLSEFADSLK 370 380 390 400 410 420 KADYVYLCDI FGSARENIGK LSIEDLREKI PQAQLIAEED TSVLKAHKDG ILIFMGAGDI 430 QKYLRAYEKV AV

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAFR-032.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000813 Genomic DNA. Translation: ABV63284.1.
RefSeq	YP_001487844.1. NC_009848.1.
3D structure databases
ProteinModelPortal	A8FGB7.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FGB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000065529; EBBACP00000063862; EBBACG00000065520.
GeneID	5621896.
GenomeReviews	Gene locus BPUM_2626 in contig CP000813_GR.
KEGG	bpu:BPUM_2626.
PATRIC	18969190. VBIBacPum16546_2689.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0773.
GeneTree	EBGT00050000000630.
HOGENOM	HBG476594.
OMA	YIDDYAH.
ProtClustDB	PRK00421.
Enzyme and pathway databases
BioCyc	BPUM315750:BPUM_2626-MONOMER.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01924.
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. MurC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BACP2

Accession

Primary (citable) accession number: A8FGB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program