A8FFB1 (SYGA_BACP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	BPUM_2259

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm HAMAP MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Glycine--tRNA ligase alpha subunit HAMAP MF_00254		PRO_1000059050

Sequences

	Sequence		Length	Mass (Da)
	A8FFB1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: B6539AB55F40DEBE	FASTA	295	34,116
	10 20 30 40 50 60 MNIQDMILTL QKHWSNEGCV LMQAYDTEKG AGTMSPYTFL RSIGPEPWKV AYVEPSRRPA 70 80 90 100 110 120 DGRYGENPNR LYQHHQFQVI IKPSPDNIQE LYLESLKALG IDPLKHDIRF VEDNWENPSL 130 140 150 160 170 180 GCAGLGWEVW LDGMEITQFT YFQQVGGLEC KPVSVEITYG IERLASYIQD KENVFDLEWT 190 200 210 220 230 240 DGFTVRDLFL MAEYEHSVYT FETSNTDMLF ELFTTYEKEA HSQMDKGLVH PAYDYVLKCS 250 260 270 280 290 HTFNLLDAKG AISVTERTGY IGRVRQLARK VAKTYYEERE KLGFPMLKEE AASHE

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAFR-032.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000813 Genomic DNA. Translation: ABV62928.1.
RefSeq	YP_001487488.1. NC_009848.1.
3D structure databases
ProteinModelPortal	A8FFB1.
SMR	A8FFB1. Positions 1-281.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FFB1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000064631; EBBACP00000062964; EBBACG00000064622.
GeneID	5621529.
GenomeReviews	Gene locus BPUM_2259 in contig CP000813_GR.
KEGG	bpu:BPUM_2259.
PATRIC	18968428. VBIBacPum16546_2309.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0752.
GeneTree	EBGT00050000002437.
HOGENOM	HBG285316.
OMA	CRRPTDG.
ProtClustDB	PRK09348.
Enzyme and pathway databases
BioCyc	BPUM315750:BPUM_2259-MONOMER.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
KO	K01878.
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. GlyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_BACP2

Accession

Primary (citable) accession number: A8FFB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program