A8FF41 (GCST_BACP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	BPUM_2189

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Aminomethyltransferase HAMAP MF_00259		PRO_1000059081

Sequences

	Sequence		Length	Mass (Da)
	A8FF41 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 0464AB8AFA9DF2C6	FASTA	365	40,306
	10 20 30 40 50 60 MLKRTPLFHA YETFGAKTID FGGWELPVQF SSIKEEHEAV RTKAGLFDVS HMGEVEIKGQ 70 80 90 100 110 120 DALPFLQRLL TNDVSKLTDG KALYTAMCYE DGGTVDDLLV YQKEKNDYLL VINASNIEKD 130 140 150 160 170 180 VEWLLQHQGE NDVLIQNVSD EIALLALQGP LAADIMKDVA DEEVTSLKPF TFLSKAEVAQ 190 200 210 220 230 240 KEVLVSRTGY TGEDGFEIYC QSEDAVHIWS ALLKVGAPKG LIPCGLGARD TLRFEARLPL 250 260 270 280 290 300 YGQELTKDIS PLEGGIGFAV KTDKEANFIG KEALKKQKEE GPKRKLVGIE MIDKGIPRTD 310 320 330 340 350 360 YPVFSGEKQI GVVTTGTQSP TLKKNVGLAL IESSQAQLGT VVEVQVRKKR LKAKIVATPF YKRAK

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAFR-032.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000813 Genomic DNA. Translation: ABV62858.1.
RefSeq	YP_001487418.1. NC_009848.1.
3D structure databases
ProteinModelPortal	A8FF41.
SMR	A8FF41. Positions 1-363.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FF41.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000063627; EBBACP00000061960; EBBACG00000063618.
GeneID	5621458.
GenomeReviews	Gene locus BPUM_2189 in contig CP000813_GR.
KEGG	bpu:BPUM_2189.
PATRIC	18968280. VBIBacPum16546_2236.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0404.
GeneTree	EBGT00050000001932.
HOGENOM	HBG299834.
OMA	VINASNK.
Enzyme and pathway databases
BioCyc	BPUM315750:BPUM_2189-MONOMER.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. [Graphical view]
KO	K00605.
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. GcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_BACP2

Accession

Primary (citable) accession number: A8FF41

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program