Reviewed, UniProtKB/Swiss-Prot A8FF40 (GCSPA_BACP2)

Names and origin

Protein names

Recommended name:
    Probable glycine dehydrogenase [decarboxylating] subunit 1
    EC=1.4.4.2
Alternative name(s):
    Glycine decarboxylase subunit 1
    Glycine cleavage system P-protein subunit 1

Gene names

Name:	gcvPA
Ordered Locus Names:	BPUM_2188

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity.
Catalytic activity	Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO2. HAMAP MF_00712
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is an heterodimer of two subunits By similarity.
Sequence similarities	Belongs to the gcvP family. N-terminal subunit subfamily.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycine dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Probable glycine dehydrogenase [decarboxylating] subunit 1 HAMAP MF_00712		PRO_1000062079

Sequences

	Sequence		Length	Mass (Da)
	A8FF40-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 960C81617A212877	FASTA	448	49,168
	10 20 30 40 50 60 MKHRYLPQTE QDQKEMLDVI GVQSIDELFS DIPEKVRFKG AYNIKPAASE TELVRELSQL 70 80 90 100 110 120 AAKNKDTVSY ASFLGAGVYD HYQPVIVDHV ISRSEFYTAY TPYQPEISQG ELQAIFEFQT 130 140 150 160 170 180 MIAELTGMDL ANSSMYDGGT ALAEAAMLAA GHTKKKKVVV SETVHPEARA VLKTYAKGQH 190 200 210 220 230 240 IEVVEVPAKK GQTDLAALEK AVCDETAAVL VQYPNFFGVV EPLKDIEPIA HKGKSLFVVS 250 260 270 280 290 300 SNPLALGLLT PPGKLGADIV VGDAQPFGIP AAFGGPHCGY FAVTKKLMRK VPGRLVGQTE 310 320 330 340 350 360 DENGVRGFVL TLQAREQHIR RDKATSNICS NQALNALAAS VAMTALGKTG IKDIAYQNVQ 370 380 390 400 410 420 KAHYAKTQAE TYGLLADVEG THFNEFVIKL QEPVQEVNKR LLEKGIIGGY DLGRDYPELQ 430 440 HHMLVAVTEL RTKEEIDTFI KELGDRHE

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000813 Genomic DNA. Translation: ABV62857.1.
RefSeq	YP_001487417.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5621457.
GenomeReviews	Gene locus BPUM_2188 in contig CP000813_GR.
KEGG	bpu:BPUM_2188.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8FF40. VANASMY.
Family and domain databases
HAMAP	MF_00712. [Tree]
InterPro	IPR003437. GDC-P. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11773. GDC-P. 1 hit.
Pfam	PF02347. GDC-P. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCSPA_BACP2

Accession

Primary (citable) accession number: A8FF40

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	June 16, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)