Reviewed, UniProtKB/Swiss-Prot A8FF11 (DXS_BACP2)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	BPUM_2159

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity.
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 633	633	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000059444

Sequences

	Sequence		Length	Mass (Da)
	A8FF11-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: F63C868DE5575F50	FASTA	633	69,494
	10 20 30 40 50 60 MDLLSIKNPT FLKGMSNAEL EELSADIRMF LIESLANSGG HIGPNLGVVE LTVALHKVFD 70 80 90 100 110 120 SPKDKFLWDV GHQSYVHKLL TGRGSEFDTL RQYKGLCGFP KRNESEHDVW ETGHSSTSLS 130 140 150 160 170 180 GAMGMAAARD IKGTDEYILP IIGDGALTGG MALEALNHIG DEKKDMIVIL NDNEMSIAPN 190 200 210 220 230 240 VGAIHTMLGR LRTAGKYQWV KDELEYLFKR IPAVGGKLAA TAERIKDSLK YLLVSGMFFE 250 260 270 280 290 300 EMGFTYLGPV DGHSYEDLFE NLEYAKKTKG PVLLHVITKK GKGYQPAESD KIGTWHGTGP 310 320 330 340 350 360 YKIDTGDFVK PAAAAPSWSG LVSETVRKLA REDERIVAIT PAMPVGSKLE GFAKEFPERM 370 380 390 400 410 420 FDVGIAEQHA ATMAAGLATQ NMKPFLAIYS TFLQRAYDQV LHDICRQNLN VFIGIDRAGL 430 440 450 460 470 480 VGADGETHQG VFDIAFMRHM PNMVLMMPKD ENEGQHMVNT AIQYDDGPIA MRFPRGNGLG 490 500 510 520 530 540 VKMDEQLKTI PIGSWEVLHP GKDAVILTFG TTIKMALQAA EELQKEGKSV RVVNARFIKP 550 560 570 580 590 600 LDEAMLNDIL SEGIPILTIE EAVLQGGFGS SVLEYIHDKK ASHIKVERMG IPDEFIEHGS 610 620 630 VDALLEEIGL TKVQVADTLR DMLPVAPRKG IGS

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000813 Genomic DNA. Translation: ABV62828.1.
RefSeq	YP_001487388.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8FF11.
Genome annotation databases
GeneID	5621428.
GenomeReviews	Gene locus BPUM_2159 in contig CP000813_GR.
KEGG	bpu:BPUM_2159.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QRFPDRY.
Family and domain databases
HAMAP	MF_00315. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BACP2

Accession

Primary (citable) accession number: A8FF11

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)