Reviewed, UniProtKB/Swiss-Prot A8FEW4 (NAMA_BACP2)

Names and origin

Protein names

Recommended name:
    NADPH dehydrogenase
    EC=1.6.99.1
Alternative name(s):
    Xenobiotic reductase

Gene names

Name:	namA
Ordered Locus Names:	BPUM_2112

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity.
Catalytic activity	NADPH + acceptor = NADP(+) + reduced acceptor.
Cofactor	FMN By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	NADPH dehydrogenase		PRO_1000069456
Regions
	Nucleotide binding	23 – 27	5	FMN By similarity
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	215	1	FMN By similarity
	Binding site	308	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FEW4-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 55640EE0A00E7407	FASTA	338	37,484
	10 20 30 40 50 60 METKLFSPWT LKGVTLKNRI VMSPMCMYSS YDRDGKLQPF HFTHYISRAQ GQAGLIMVEA 70 80 90 100 110 120 SAVSPEGRIS DQDLGIWSDE HIEGFASLNE QIKAYGTKTA IQLAHAGRKA ELDGDILAPS 130 140 150 160 170 180 SIPFDEQSKT PVQMSVDQIK DTVQAFQDAA VRAKKAGFDI IEIHGAHGYL INEFLSPLAN 190 200 210 220 230 240 HRTDDYGGSP ENRYRFLREV VNAVNEVWDG PLFVRVSASD YTTKGLDIAD YIGIATWLKE 250 260 270 280 290 300 QGVDLIDVSS GAVVQARIST FPGYQVTFAE KIKEGAGIQT GAVGLITSGV QAEEILRNQR 310 320 330 ADLIFIGREF LRDPYFPKTA AEELRTSIEG PRQYDRAW

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000813 Genomic DNA. Translation: ABV62781.1.
RefSeq	YP_001487341.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5621381.
GenomeReviews	Gene locus BPUM_2112 in contig CP000813_GR.
KEGG	bpu:BPUM_2112.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01614. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_BACP2

Accession

Primary (citable) accession number: A8FEW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	November 25, 2008
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)