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Protein

Processive diacylglycerol beta-glucosyltransferase

Gene

ugtP

Organism
Bacillus pumilus (strain SAFR-032)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Processive glucosyltransferase involved in the biosynthesis of both the bilayer- and non-bilayer-forming membrane glucolipids. Is able to successively transfer up to three glucosyl residues to diacylglycerol (DAG), thereby catalyzing the formation of beta-monoglucosyl-DAG (3-O-(beta-D-glucopyranosyl)-1,2-diacyl-sn-glycerol), beta-diglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol) and beta-triglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol). Beta-diglucosyl-DAG is the predominant glycolipid found in Bacillales and is also used as a membrane anchor for lipoteichoic acid (LTA).UniRule annotation

Catalytic activityi

UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP.
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP.
UDP-glucose + 1,2-diacyl-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol.UniRule annotation

Pathwayi: diglucosyl-diacylglycerol biosynthesis

This protein is involved in the pathway diglucosyl-diacylglycerol biosynthesis, which is part of Glycolipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway diglucosyl-diacylglycerol biosynthesis and in Glycolipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciBPUM315750:G1G9U-1981-MONOMER
UniPathwayiUPA00894

Protein family/group databases

CAZyiGT28 Glycosyltransferase Family 28

Names & Taxonomyi

Protein namesi
Recommended name:
Processive diacylglycerol beta-glucosyltransferaseUniRule annotation (EC:2.4.1.315)
Alternative name(s):
Beta-diglucosyldiacylglycerol synthaseUniRule annotation
Short name:
Beta-DGSUniRule annotation
Short name:
DGlcDAG synthaseUniRule annotation
Short name:
Glc2-DAG synthaseUniRule annotation
Beta-gentiobiosyldiacylglycerol synthaseUniRule annotation
Beta-monoglucosyldiacylglycerol synthaseUniRule annotation
Short name:
Beta-MGSUniRule annotation
Short name:
MGlcDAG synthaseUniRule annotation
Beta-triglucosyldiacylglycerol synthaseUniRule annotation
Short name:
TGlcDAG synthaseUniRule annotation
Diglucosyl diacylglycerol synthase (1,6-linking)
Glucosyl-beta-1,6-glucosyldiacylglycerol synthaseUniRule annotation
UDP glucosyltransferaseUniRule annotation
UDP-glucose:1,2-diacylglycerol-3-beta-D-glucosyltransferaseUniRule annotation
Gene namesi
Name:ugtPUniRule annotation
Ordered Locus Names:BPUM_1928
OrganismiBacillus pumilus (strain SAFR-032)
Taxonomic identifieri315750 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001355 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000674101 – 383Processive diacylglycerol beta-glucosyltransferaseAdd BLAST383

Proteomic databases

PRIDEiA8FED1

Interactioni

Protein-protein interaction databases

STRINGi315750.BPUM_1928

Structurei

3D structure databases

ProteinModelPortaliA8FED1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 28 family. UgtP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108QBV Bacteria
COG0707 LUCA
HOGENOMiHOG000221565
KOiK03429
OMAiEPAGHIV
OrthoDBiPOG091H02I5

Family and domain databases

HAMAPiMF_01280 Diacylglyc_glucosyltr, 1 hit
InterProiView protein in InterPro
IPR009695 Diacylglyc_glucosyltr_N
IPR001296 Glyco_trans_1
IPR023589 Pro_diacylglycrl_glcsylTrfase
PfamiView protein in Pfam
PF00534 Glycos_transf_1, 1 hit
PF06925 MGDG_synth, 1 hit

Sequencei

Sequence statusi: Complete.

A8FED1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTNKKILIL TANYGNGHMQ VAKTLYDECK SQGFEHVVVS NLYQESNPIV
60 70 80 90 100
SEVTQYLYLK SFSIGKQFYR LFYYGVDKIY NKRKFNIYLK MGNKRLDELI
110 120 130 140 150
QLHNPDIIII TFPMIVVPEY RNKTGKIIPT FNVMTDFCLH KIWVHENIDR
160 170 180 190 200
YYVATDYVKQ KLVEIGTHPS DVKVTGIPIR PQFEADVPKS KIYKKYGLSS
210 220 230 240 250
NKKVLLIMAG AHGVLKNVKE LCEALLLDSE VQIVVVCGKN AALKQSLSDL
260 270 280 290 300
EQTHPDQLKA LGYVEQIDEL FRVTDCMITK PGGITLTEAT ALGVPVILYK
310 320 330 340 350
PVPGQEKENA HFFEDYGAAI VINRHEDILE SVTNLLQDEE KLESMKQNMK
360 370 380
SLHLKHSSQT ILEDIVEQSD LITNNKTYAR ALS
Length:383
Mass (Da):43,680
Last modified:November 13, 2007 - v1
Checksum:i2DD85F41BACE9D98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000813 Genomic DNA Translation: ABV62598.1
RefSeqiWP_012010316.1, NC_009848.4

Genome annotation databases

EnsemblBacteriaiABV62598; ABV62598; BPUM_1928
KEGGibpu:BPUM_1928

Similar proteinsi

Entry informationi

Entry nameiUGTP_BACP2
AccessioniPrimary (citable) accession number: A8FED1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: February 28, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome