A8FEC9 (FTHS_BACP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	BPUM_1926

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_0000318564
Regions
	Nucleotide binding	69 – 76	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FEC9 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 06EBAA5FB9E818FB	FASTA	560	60,467
	10 20 30 40 50 60 MITKHLSDID IAQQAKLRPI QDIAAKLQLH DEELECYGFT KAKISLTIFD RLKEQKEGHV 70 80 90 100 110 120 ILVTSINPTP AGEGKSTVTV GLGQAFEKIG KKAIIAMREP SLGPTMGIKG GAAGGGYSQV 130 140 150 160 170 180 LPMEEINLHF TGDFHAITSA HNALSAFIDN HIHHGNELDI DARRIVWKRV LDLNDRALRQ 190 200 210 220 230 240 VVVGLGGQVN GYPREDGFDI TVASEMMAIL CLAEDLTDLK KRLSSIVVAY NRDGEPVTVG 250 260 270 280 290 300 ALGYEGVLTL LLKDALKPNL VQTIEGTPAL VHGGPFANIA HGCNSLIATK MAAKLADYVV 310 320 330 340 350 360 TEAGFGADLG AEKFLNIKTR AGDFKPGAVV IVATVRALKM HGGMKKTELK EENTQAVLKG 370 380 390 400 410 420 IHNLEKHIET VQAFGLPYIV AVNRFITDTK EEIQAIEDWC LAHDHPVKAV NVWEEGGEGG 430 440 450 460 470 480 TALAEELTTL IEKKKNSFSY LYEEDDSIEE KLSKVAKVVY GADGVTLTSK ARKQLAAIEE 490 500 510 520 530 540 NSWGHLPVCM AKTQYSLSDD PALIGRPKGF TITIRELKPS VGAGFIVALT GSILTMPGLP 550 560 KKPAALEMDL LEDGSVTGLF

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAFR-032.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000813 Genomic DNA. Translation: ABV62596.1.
RefSeq	YP_001487156.1. NC_009848.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8FEC9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000063362; EBBACP00000061695; EBBACG00000063353.
GeneID	5621194.
GenomeReviews	Gene locus BPUM_1926 in contig CP000813_GR.
KEGG	bpu:BPUM_1926.
PATRIC	18967748. VBIBacPum16546_1970.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
GeneTree	EBGT00050000004414.
HOGENOM	HBG677721.
OMA	EIMAVLC.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	BPUM315750:BPUM_1926-MONOMER.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_BACP2

Accession

Primary (citable) accession number: A8FEC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program