Reviewed, UniProtKB/Swiss-Prot A8FEC5 (ILVD_BACP2)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BPUM_1922

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000057095
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A8FEC5-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: B9962FCD74939B91	FASTA	558	59,704
	10 20 30 40 50 60 MAELRSNMIK QGIDRAPHRS LLRAAGVKDE DFGKPFIAVC NSYIDIVPGH VHLQEFGKIV 70 80 90 100 110 120 KEAIREAGGV PFEFNTIGVD DGIAMGHIGM RYSLPSREII ADSVETVVSA HWFDGMVCIP 130 140 150 160 170 180 NCDKITPGMM MAAMRVNIPT VFVSGGPMEA GRTSDGRKIS LSSVFEGVGA YQSGKINEEE 190 200 210 220 230 240 LNELEQFGCP TCGSCSGMFT ANSMNCLAEA LGIALPGNGT ILATSPERRE FAKKSAKQLM 250 260 270 280 290 300 ELIKKDIKPR DIVTEKAIDN AFALDMALGG STNTVLHTLA IANEAGVEYS LERINEVAER 310 320 330 340 350 360 VPHLSKLAPA SDVYIEDLHE AGGVTAALNE LSKKEGALHL DTMTVTAKTL GENIAGHEVK 370 380 390 400 410 420 DYNVIYPIDK PFTEKGGLAV LFGNLAPDGA IIKTGGVQDG ITRHEGPAIV FESQEEALEG 430 440 450 460 470 480 IINRKVEAGH VVIIRYEGPK GGPGMPEMLA PTSQIVGMGL GPKVALITDG RFSGASRGLS 490 500 510 520 530 540 IGHVSPEAAE GGPLAFVENG DHVVVDIEQR ILSVDVPEEE WEKRKANWKG FEPKVKTGYL 550 ARYSKLVTSA NTGGIMKI

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000813 Genomic DNA. Translation: ABV62592.1.
RefSeq	YP_001487152.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8FEC5.
Genome annotation databases
GeneID	5621190.
GenomeReviews	Gene locus BPUM_1922 in contig CP000813_GR.
KEGG	bpu:BPUM_1922.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SRKVPCL.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BACP2

Accession

Primary (citable) accession number: A8FEC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)