Reviewed, UniProtKB/Swiss-Prot A8FE66 (ODO1_BACP2)

Names and origin

Protein names

Recommended name:
    2-oxoglutarate dehydrogenase E1 component
    EC=1.2.4.2
Alternative name(s):
    Alpha-ketoglutarate dehydrogenase

Gene names

Name:	odhA
Ordered Locus Names:	BPUM_1862

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	944 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.
Catalytic activity	2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO(2).
Cofactor	Thiamine pyrophosphate By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the alpha-ketoglutarate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxoglutarate dehydrogenase (succinyl-transferring) activity Inferred from electronic annotation. Source: HAMAP thiamin pyrophosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 944	944	2-oxoglutarate dehydrogenase E1 component		PRO_1000065697

Sequences

	Sequence		Length	Mass (Da)
	A8FE66-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 5CF1400B2F45876F	FASTA	944	106,822
	10 20 30 40 50 60 MFQNDVKQPL SWEEFHGPNL GYVLELYDQY VQDPTSVDED LRGIFDELGA PPSEMKEEIG 70 80 90 100 110 120 KKENSVVTSE QIQKIASVVK LAEDIRTYGH LNASVNPLRK EKELQELFPL KEYGLTEEDV 130 140 150 160 170 180 KNIPISIISP DAPKHISNGI EAINHLRNTY KRTISFEFDH VHDFEERNWL SKSIESGELF 190 200 210 220 230 240 KKKPADKLVS VFKRLTEVEQ FEQFLHKTFV GQKRFSIEGL DALVPVLDEI ISESVTQGTS 250 260 270 280 290 300 NINIGMAHRG RLNVLAHVLG KPYEIIFSEF QHAPNKELVP SEGSIGISYG WTGDVKYHLG 310 320 330 340 350 360 ADRQIKDEDT KSARVTLANN PSHLEFIDPI IEGSTRAAQE LRTQKGYPAQ DVEKALAILI 370 380 390 400 410 420 HGDAAFPGEG IVAETLNLSQ LVGYQVGGTI HIIANNMIGF TTESNESRST KYASDLAKGF 430 440 450 460 470 480 EIPIVHVNAD DPEACLAAVQ LAVEYRKRFK KDFLIDLIGY RRYGHNEMDE PSTTQPMLYD 490 500 510 520 530 540 AVRKHKTVKN IFADKLVSEG LLTKEQREEI EQAVATRIEE AYQKVPSKKE HTIQEIELPE 550 560 570 580 590 600 PVSNGFPAVD TSVEFDVLRK LNEELISWPD DFQVFGKLKR ILEKRAKVFT DDRKVEWSLG 610 620 630 640 650 660 EALAFASILK DGTPIRMTGQ DSERGTFAQR NLVLHDSQTG NEFIALHELS DANASFTVHN 670 680 690 700 710 720 SPLSEGSVIG FEYGYNVYSP ETLVIWEAQF GDFANAAQVY FDQFISAGRA KWGQKSGLVM 730 740 750 760 770 780 LLPHGYEGQG PEHSSGRTER FLQSAAENNW TVANLTSAAQ YFHILRRQAK MLLREEIRPL 790 800 810 820 830 840 VIMTPKSLLR NPNSLSEVQE LTDGQFQPVL EQPGLVHDHE KVSRLVLSSG KVSIDISDRF 850 860 870 880 890 900 TQMEEPKDWL HIARVEQLYP FPAKDIKAIL SKLTNLEEIV WTQEEPQNMG AWGYIEPYLR 910 920 930 940 EIAPEKVKVR YIGRRRRSST AEGDPTVHKK EQERIVSDSL TRKN

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000813 Genomic DNA. Translation: ABV62533.1.
RefSeq	YP_001487093.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5621130.
GenomeReviews	Gene locus BPUM_1862 in contig CP000813_GR.
KEGG	bpu:BPUM_1862.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01169. [Tree]
InterPro	IPR011603. 2oxoglutarate_DHase_E1. IPR001017. DHase_E1. IPR005475. Transketo_Cen_R. [Graphical view]
PANTHER	PTHR23152. 2oxoglutarate_DH_E1. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. [Graphical view]
PIRSF	PIRSF000157. Oxoglu_dh_E1. 1 hit.
TIGRFAMs	TIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODO1_BACP2

Accession

Primary (citable) accession number: A8FE66

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	November 4, 2008
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)