Reviewed, UniProtKB/Swiss-Prot A8FE33 (XYLA_BACP2)

Names and origin

Protein names

Recommended name:
    Xylose isomerase
    EC=5.3.1.5

Gene names

Name:	xylA
Ordered Locus Names:	BPUM_1829

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-xylose = D-xylulose. HAMAP MF_00455
Cofactor	Binds 2 magnesium ions per subunit By similarity. HAMAP MF_00455
Subunit structure	Homotetramer By similarity. HAMAP MF_00455
Subcellular location	Cytoplasm By similarity HAMAP MF_00455.
Sequence similarities	Belongs to the xylose isomerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Pentose shunt Xylose metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	D-xylose metabolic process Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP xylose isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Xylose isomerase HAMAP MF_00455		PRO_1000060318
Sites
	Active site	107	1	By similarity
	Active site	110	1	By similarity
	Metal binding	238	1	Magnesium 1 By similarity
	Metal binding	274	1	Magnesium 1 By similarity
	Metal binding	274	1	Magnesium 2 By similarity
	Metal binding	277	1	Magnesium 2 By similarity
	Metal binding	302	1	Magnesium 1 By similarity
	Metal binding	313	1	Magnesium 2 By similarity
	Metal binding	315	1	Magnesium 2 By similarity
	Metal binding	345	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FE33-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 379343C01EDB2C87	FASTA	445	50,416
	10 20 30 40 50 60 MANSNMKTLH SLEGIGKIQF EGKQSKNPLA FKSYNPSEVI GGKTMKDHLR FSVAYWHTLT 70 80 90 100 110 120 ADGTDMFGTG TMQRAWDSYS GMDLAKARLE VAFQLFDLLD VPYFSFHDRD IAPEGDTLQE 130 140 150 160 170 180 TNQHLDIILE RMKEYMRDSG VKLLWNTANM FKHPRFVHGA ATSCHADVFA YAAAQVKKGI 190 200 210 220 230 240 ETAKELRAEN YVFWGGREGY DTLLNTDMKR ELDHMASFLH MAVDYAKEIG YTGQFLIEPK 250 260 270 280 290 300 PKEPTTHQYD ADAATSIAFL KQYGLDTHFK LNIEANHATL AGHTFEHELR VARIHGLLGS 310 320 330 340 350 360 VDANQGNTLL GWDTDEFPTD LYATTLAMYE ILQNGGLGTG GLNFDAKVRR ASFELEDILY 370 380 390 400 410 420 AHIAGMDAFA KGLRVAHRLI EDRVFEDVIK HRYRSFSEGI GRDISEGKAN FHILEEYALK 430 440 HPLIRNESGR EEQLKARLNQ YLLED

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000813 Genomic DNA. Translation: ABV62500.1.
RefSeq	YP_001487060.1.
3D structure databases
SMR	A8FE33. Positions 12-444.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FE33.
Genome annotation databases
GeneID	5621096.
GenomeReviews	Gene locus BPUM_1829 in contig CP000813_GR.
KEGG	bpu:BPUM_1829.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2115.
HOGENOM	HBG297199.
OMA	LLGWDTD.
Family and domain databases
HAMAP	MF_00455. Xylose_isom_A. [Tree]
InterPro	IPR013022. Xyl_isomerase-like_TIM-brl. IPR012307. Xyl_isomerase_TIM-brl. IPR013452. Xylose_isom_bac. IPR001998. Xylose_isomerase. [Graphical view]
Gene3D	G3DSA:3.20.20.150. Xyl_isomerase-like_TIM-brl. 1 hit.
Pfam	PF01261. AP_endonuc_2. 1 hit. [Graphical view]
PRINTS	PR00688. XYLOSISMRASE.
TIGRFAMs	TIGR02630. xylose_isom_A. 1 hit.
PROSITE	PS51415. XYLOSE_ISOMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLA_BACP2

Accession

Primary (citable) accession number: A8FE33

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	February 9, 2010
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)