Reviewed, UniProtKB/Swiss-Prot A8FDV4 (IOLA_BACP2)

Names and origin

Protein names

Recommended name:
    Methylmalonate semialdehyde dehydrogenase [acylating]
      Short name=MMSA dehydrogenase
      Short name=MMSDH
      Short name=MSDH
    EC=1.2.1.27
Alternative name(s):
    Malonate semialdehyde dehydrogenase [acetylating]
      Short name=MSA dehydrogenase
    EC=1.2.1.18

Gene names

Name:	iolA
Ordered Locus Names:	BPUM_1749

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively By similarity.
Catalytic activity	3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. HAMAP MF_01670 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. HAMAP MF_01670
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. HAMAP MF_01670
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. IolA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Methylmalonate semialdehyde dehydrogenase [acylating] HAMAP MF_01670		PRO_0000352331
Regions
	Nucleotide binding	178 – 182	5	NAD By similarity
Sites
	Active site	286	1	Nucleophile By similarity
	Binding site	386	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FDV4-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 418DF7891FAED79E	FASTA	486	53,208
	10 20 30 40 50 60 MTKTDVQMLK NYIGGQWIEA ETSQTEAVYN PATGKIIAEV PLSTKKDVER AVQAAQEAFT 70 80 90 100 110 120 TWSKTPVPRR ARILFKYQQL LVDKWDELAE LVTMENGKSI TEAKGEVQRG IECVEFAAGA 130 140 150 160 170 180 PTLMMGKQLP DIASGLESGM YRYPIGVIGG ITPFNFPMMV PCWMFPLAIA CGNTFVLKPS 190 200 210 220 230 240 ERTPILAARL AELFEEAGLP KGVLNIVNGA HDVVNGLLEH QKVKAISFVG SQPVAEYVYK 250 260 270 280 290 300 KGTEHGKRVQ ALAGAKNHSI VLKDADLDAA TKQIIGAAFG SAGERCMAAA VVAVEEEVAD 310 320 330 340 350 360 DLIQKLVDES NELVIGNGIN EEVFLGPVIR EEHKERTLQY IQSGIEEGAS LIRDGRKDHE 370 380 390 400 410 420 TNGKGYFVGP TIFDHVTNQM KIWQDEIFAP VLSIVRVSSL AEAIDLSNQS KFANGACLYT 430 440 450 460 470 480 DSASSIREFR ENIEAGMLGV NIGVPAPMAF FPFSGWKDSF YGDLHANGTD GVEFYTRKKM VTARYM

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000813 Genomic DNA. Translation: ABV62421.1.
RefSeq	YP_001486981.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5621016.
GenomeReviews	Gene locus BPUM_1749 in contig CP000813_GR.
KEGG	bpu:BPUM_1749.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8FDV4. RENIEAG.
Family and domain databases
HAMAP	MF_01670. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLA_BACP2

Accession

Primary (citable) accession number: A8FDV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	November 13, 2007
Last modified:	June 16, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)