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A8FD76 (A8FD76_BACP2) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Name:topA EMBL ABV62193.1
Ordered Locus Names:BPUM_1510
OrganismBacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]
Taxonomic identifier315750 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length691 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
A8FD76 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: BF2F70D923F2DF01

FASTA69179,239
        10         20         30         40         50         60 
MADYLVIVES PAKAKTIERY LGKKYKVKAS MGHIRDLPKS QLGVDTEHDF EPRYITIRGK 

        70         80         90        100        110        120 
GPVLKELKTA AKKAKKVYLA ADPDREGEAI AWHLAHSLDL DLSSDCRVVF NEITKDAIKD 

       130        140        150        160        170        180 
SFKHPRMINM DLVDAQQARR ILDRLVGYKI SPILWKKVKK GLSAGRVQSV ALRLIIDREN 

       190        200        210        220        230        240 
EINEFKPEEY WTIDGTFLKG KESFEASFFG VNGKKHPLKT KEDVKEILSK LKGSKFSVEK 

       250        260        270        280        290        300 
VTKKERKRNP AVPFTTSTLQ QEAARKLNFR AKKTMMIAQQ LYEGIDLGKE GTVGLITYMR 

       310        320        330        340        350        360 
TDSTRISNTA IEEVSAFIDQ TYGKNFLNTT KRTVKKNENA QDAHEAIRPT STLRKPADVK 

       370        380        390        400        410        420 
HVLSRDQLRL YKLIWERFVA SQMAPAVLDT MSVDLDNNGL TFRANGSKVK FPGFMKVYVE 

       430        440        450        460        470        480 
GKDDQLEEKD KMLPDLKEGD TVLSKDIEPE QHFTQPPPRY TEARLVKTLE ELGIGRPSTY 

       490        500        510        520        530        540 
APTLDTIQKR GYVALDNKRF IPTELGEIVL NLIIEFFPEI INVEFTAKME KELDSVEEGT 

       550        560        570        580        590        600 
IEWVRIIDSF YQDFAKRVEK AEAEMQEVEI EPEYAGVDCE ECGHPMVYKM GRYGKFMACS 

       610        620        630        640        650        660 
NFPDCRNTKP IVKDIGVKCP TCHEGNIVER KSKKRRIFYG CDRFPECEFV SWDKPIERKC 

       670        680        690 
PKCENMLVEK KLKKGMQVQC VNCDYKEEQQ K

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000813 Genomic DNA. Translation: ABV62193.1.
RefSeqYP_001486753.1. NC_009848.1.

3D structure databases

ProteinModelPortalA8FD76.
ModBaseSearch...

Protein-protein interaction databases

STRINGA8FD76.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000063143; EBBACP00000061476; EBBACG00000063134.
GeneID5620777.
GenomeReviewsGene locus BPUM_1510 in contig CP000813_GR.
KEGGbpu:BPUM_1510.
PATRIC18966855. VBIBacPum16546_1524.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0550.
GeneTreeEBGT00070000032133.
HOGENOMHBG512935.
OMAEEYWSID.
ProtClustDBPRK05582.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA8FD76_BACP2
AccessionPrimary (citable) accession number: A8FD76
Entry history
Integrated into UniProtKB/TrEMBL: November 13, 2007
Last sequence update: November 13, 2007
Last modified: December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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