Reviewed, UniProtKB/Swiss-Prot A8FD15 (PYRC_BACP2)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	BPUM_1449

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00220
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00220
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Dihydroorotase HAMAP MF_00220		PRO_1000058653
Sites
	Metal binding	59	1	Zinc 1 By similarity
	Metal binding	61	1	Zinc 1 By similarity
	Metal binding	141	1	Zinc 1; via carbamate group By similarity
	Metal binding	141	1	Zinc 2; via carbamate group By similarity
	Metal binding	178	1	Zinc 2 By similarity
	Metal binding	231	1	Zinc 2 By similarity
	Metal binding	304	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	141	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FD15-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: B50A9168280389E2	FASTA	428	46,993
	10 20 30 40 50 60 MSYLIKNGFI LTSTGEKVQQ DIRVEGEVIQ AIGHLEREDG EEVIDAKGLF VSPGLIDLHV 70 80 90 100 110 120 HLREPGGEKK ETIETGSKAA ARGGYTTIAA MPNTRPVPDT KEQMEWLMNR IEETSSVRVL 130 140 150 160 170 180 PYASITTRQI GEEMTDFAAL KEAGAFAFTD DGVGIQTAGM MYEAMKKAAS LDQAIVAHCE 190 200 210 220 230 240 DNSLIYGGCV HEGEFSKANG LNGIPSICES VHIARDVLLA EAANCHYHVC HISTKESVRV 250 260 270 280 290 300 VRDAKKAGIR VTAEVSPHHL LLCDADIPGL DTNYKMNPPL RGKEDREALI EGLLDGTIDF 310 320 330 340 350 360 IATDHAPHTE EEKNETMQRA PFGIVGLETA FPLLYTRFVK TGEWTLKELV DYMTIKPAEA 370 380 390 400 410 420 FSLPYGKLEK GQIADITLID LNKEMAIDKK SFLSKGQNTP FDKLTVSGWP VMTLASGKVV YEEGRLVK

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000813 Genomic DNA. Translation: ABV62132.1.
RefSeq	YP_001486692.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8FD15.
Protein family/group databases
MEROPS	M38.972.
Genome annotation databases
GeneID	5620716.
GenomeReviews	Gene locus BPUM_1449 in contig CP000813_GR.
KEGG	bpu:BPUM_1449.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CDVHPVG.
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD000518. DHOase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_BACP2

Accession

Primary (citable) accession number: A8FD15

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)