Reviewed, UniProtKB/Swiss-Prot A8FD14 (PYRB_BACP2)

Names and origin

Protein names

Recommended name:
    Aspartate carbamoyltransferase
    EC=2.1.3.2
Alternative name(s):
    Aspartate transcarbamylase
      Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	BPUM_1448

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_1000057009

Sequences

	Sequence		Length	Mass (Da)
	A8FD14-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: CE53DBD20280BBCD	FASTA	307	34,162
	10 20 30 40 50 60 MNDLSTMSSL TKEEILQLIE EATALKKGKQ ELGLAGEFVA NLFFEPSTRT RFSFEVAEKK 70 80 90 100 110 120 LGMNVLSLDA ASTSVQKGET LYDTVKTLES IGVKACVIRD STDEYYNELI GKVGIPIINA 130 140 150 160 170 180 GDGCGQHPTQ SLLDLMTIYE EFGGFEGLTI SIHGDIKHSR VARSNAEVLS RLGATVLFSG 190 200 210 220 230 240 PASFQDEQNP HGTYVQVDEA IKQSDVVMLL RIQHERHTEK MGNEDYLSTY GLTVNRAKNM 250 260 270 280 290 300 KERAIIMHPA PVNRGVEIDD SLVESKQSRI FKQMENGVYV RMAVLKRAFQ NSKLHQKGRE SVYVVSH

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000813 Genomic DNA. Translation: ABV62131.1.
RefSeq	YP_001486691.1.
3D structure databases
SMR	A8FD14. Positions 3-289.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FD14.
Genome annotation databases
GeneID	5620715.
GenomeReviews	Gene locus BPUM_1448 in contig CP000813_GR.
KEGG	bpu:BPUM_1448.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0540.
HOGENOM	HBG579429.
OMA	YFAGPEE.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P_bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn_bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
TIGRFAMs	TIGR00670. asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_BACP2

Accession

Primary (citable) accession number: A8FD14

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	February 9, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)