A8FD12 (PYRR_BACP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional protein PyrR

Including the following 2 domains:

1. Pyrimidine operon regulatory protein
2. Uracil phosphoribosyltransferase
   Short name=UPRTase
   EC=2.4.2.9

Gene names

Name:	pyrR
Ordered Locus Names:	BPUM_1446

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus pumilus

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Regulates transcriptional attenuation of the pyrimidine nucleotide (pyr) operon by binding in a uridine-dependent manner to specific sites on pyr mRNA. This disrupts an antiterminator hairpin in the RNA and favors formation of a downstream transcription terminator, leading to a reduced expression of downstream genes By similarity. HAMAP-Rule MF_01219 Also displays a weak uracil phosphoribosyltransferase activity which is not physiologically significant By similarity. HAMAP-Rule MF_01219
Catalytic activity	UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_01219
Subunit structure	Homodimer and homohexamer; in equilibrium By similarity.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrR subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Transcription termination
Ligand	RNA-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, termination Inferred from electronic annotation. Source: HAMAP nucleoside metabolic process Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: HAMAP uracil phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 180	180	Bifunctional protein PyrR HAMAP-Rule MF_01219		PRO_1000066735
Regions
	Motif	101 – 113	13	PRPP-binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FD12 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: F80731C59ACC17E2	FASTA	180	20,085
	10 20 30 40 50 60 MEQKAVILDE QAIRRALTRI AHEMIERNKG MKDVILAGIK TRGIHLAKRL AERIEQIEGN 70 80 90 100 110 120 PVIVGELDIT LYRDDLTKKT DNQDPLVKGA DIPADINDKT LIVVDDVLFT GRTVRAAMDA 130 140 150 160 170 180 LVDVGRPSSI QLAVLVDRGH RELPIRADYI GKNIPTSKAE TVMVQLNEVD QNDLVAIYEK

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAFR-032.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000813 Genomic DNA. Translation: ABV62129.1.
RefSeq	YP_001486689.1. NC_009848.1.
3D structure databases
ProteinModelPortal	A8FD12.
SMR	A8FD12. Positions 4-179.
ModBase	Search...
Protein-protein interaction databases
STRING	315750.BPUM_1446.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV62129; ABV62129; BPUM_1446.
GeneID	5620713.
KEGG	bpu:BPUM_1446.
PATRIC	18966729. VBIBacPum16546_1461.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2065.
HOGENOM	HOG000245770.
KO	K02825.
OMA	KNVPTAR.
ProtClustDB	PRK05205.
Enzyme and pathway databases
BioCyc	BPUM315750:GH6N-1535-MONOMER.
Family and domain databases
HAMAP	MF_01219. PyrR.
InterPro	IPR000836. PRibTrfase_dom. IPR023050. PyrR. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRR_BACP2

Accession

Primary (citable) accession number: A8FD12

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program