Reviewed, UniProtKB/Swiss-Prot A8FCD2 (METE_BACP2)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BPUM_1215

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000058312
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FCD2-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: A789135CBDBC0898	FASTA	762	86,584
	10 20 30 40 50 60 MTIVKTSNLG FPRIGLNREW KKALESYWKG QTDRETLLST LDEQFLTAIK TQIDQQIDVV 70 80 90 100 110 120 PSGDFTFYDH VLDTAVMFNW IPERFRSLKD PLDTYFAIAR GTKDAVSSEM TKWFNTNYHY 130 140 150 160 170 180 IVPEYEKSTE FKLTHNKPLE AYEKVKKAFG VETKPVVLGL YTFVSLSKGY EPNEVKEIQQ 190 200 210 220 230 240 RLIPLYTQLL KELEEAGVKW VQIDEPALVT ASYEDVKAVK EIYQTITEAV PALNILLQTY 250 260 270 280 290 300 FDSVDAYEEL VTYPVEAIGL DFVHDQGRNL DQVKKHGFPK DKILAAGVID GRNIWRSDLG 310 320 330 340 350 360 ERLTFVSEVI AGAQPKEVWL QPSSSLLHVP VAKHPSEQLE EKLLNGLSYA TEKLAELTTL 370 380 390 400 410 420 KEGLTKGAAA IEAAINEAEQ ALLTLKEFAK GTNADFTAER NNLSSKDFKR PVAFEERLRI 430 440 450 460 470 480 QNESLELPLL PTTTIGSFPQ SAEVRSARQK WRKNEWTNAE YDEFIKKETK RWIDIQEEIG 490 500 510 520 530 540 LDVLVHGEFE RTDMVEYFGE KLAGFAFTKF AWVQSYGSRC VKPPIIYGDV EFTAPMTVKE 550 560 570 580 590 600 TVYAQSLTEK KVKGMLTGPV TILNWSFPRT DISRKDIAFQ IAFALRKEVE ALEEAGIQVI 610 620 630 640 650 660 QVDEPALREG LPLKESDWAD YLNWAAESFR LSTSSVQNET QIHTHMCYSN FEDIVDTIED 670 680 690 700 710 720 LDADVITIEH SRSHGGFLDY LEKHPYLKGL GLGVYDIHSP RVPSVEEMSQ IIDDALNVCP 730 740 750 760 TDRFWVNPDC GLKTRQEPET IAALKNMVTA AEVARKKLAQ HA

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000813 Genomic DNA. Translation: ABV61899.1.
RefSeq	YP_001486459.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8FCD2.
Genome annotation databases
GeneID	5620482.
GenomeReviews	Gene locus BPUM_1215 in contig CP000813_GR.
KEGG	bpu:BPUM_1215.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CSLLHTP.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BACP2

Accession

Primary (citable) accession number: A8FCD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)