A8FBW1 (ARGB_BACP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	BPUM_1044

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Acetylglutamate kinase HAMAP MF_00082_B		PRO_1000057538
Regions
	Region	41 – 42	2	Substrate binding By similarity
Sites
	Binding site	63	1	Substrate By similarity
	Binding site	156	1	Substrate By similarity
	Site	8	1	Transition state stabilizer By similarity
	Site	215	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FBW1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: BA8D091D4C41E770	FASTA	258	27,738
	10 20 30 40 50 60 MDKTIVFKCG GSVIRELSDT FYENVRSLQA AGFKLAIVHG GGPEITNMLQ KLDVKTEFVD 70 80 90 100 110 120 GQRKTTKPVL EVAEMVLSGT VNKYFVSELA KHDISSVGVS GKDGQMLVAD FLDQDVYGYV 130 140 150 160 170 180 GEIKEVHPEM AEALMEKQFI PVIAPLSMTE ECQTLNVNAD LAASAVAGAM KADKLMFVTD 190 200 210 220 230 240 VEGILKNGEL LDVVTEQEAL TLIDEGIISG GMIPKVQSAL SALSGDVDEV MIVNGKGSIF 250 TGETFKGTRI VKEKEAVL

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAFR-032.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000813 Genomic DNA. Translation: ABV61728.1.
RefSeq	YP_001486288.1. NC_009848.1.
3D structure databases
ProteinModelPortal	A8FBW1.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FBW1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000065945; EBBACP00000064278; EBBACG00000065936.
GeneID	5620310.
GenomeReviews	Gene locus BPUM_1044 in contig CP000813_GR.
KEGG	bpu:BPUM_1044.
PATRIC	18965923. VBIBacPum16546_1059.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0548.
GeneTree	EBGT00050000001971.
HOGENOM	HBG497643.
OMA	GGNAMID.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	BPUM315750:BPUM_1044-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_BACP2

Accession

Primary (citable) accession number: A8FBW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program