Reviewed, UniProtKB/Swiss-Prot A8FBM6 (HEMH_BACP2)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	BPUM_0959

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Ferrochelatase HAMAP MF_00323		PRO_1000072022
Sites
	Metal binding	183	1	Iron By similarity
	Metal binding	264	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FBM6-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 08AE49295AAFAA5D	FASTA	312	35,443
	10 20 30 40 50 60 MEKKKMGLLV MAYGTPYKEE DIERYYTHIR RGRKPEPEML QDLKDRYKAI GGISPLSKIT 70 80 90 100 110 120 EQQAHGLCEH LNDIQDDIEF HVYIGLKHIE PFIEDAVADM HKDGITEAVS IVLAPHFSTF 130 140 150 160 170 180 SVKSYNKRAQ DEADKLGNLQ ITSVESWYDE PKFVDYWVKQ VKDTYASMSQ EERDSAVLIV 190 200 210 220 230 240 SAHSLPEKII AAGDPYPDQL AQSAKMIAEG AGIEHYEIGW QSEGNTPDPW LGPDVQDLTR 250 260 270 280 290 300 DLFEQKGYQT FVYVPVGFVA DHLEVLYDND YECKVVTDEI GAAYYRPEMP NAKPAFIDAL 310 ATVVLKKLDE SK

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000813 Genomic DNA. Translation: ABV61643.1.
RefSeq	YP_001486203.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8FBM6.
Genome annotation databases
GeneID	5620225.
GenomeReviews	Gene locus BPUM_0959 in contig CP000813_GR.
KEGG	bpu:BPUM_0959.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VKWLEPS.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BACP2

Accession

Primary (citable) accession number: A8FBM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)