Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A8FA55 (DDL_BACP2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:BPUM_0427
OrganismBacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]
Taxonomic identifier315750 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000057320

Regions

Domain139 – 344206ATP-grasp
Nucleotide binding172 – 22756ATP By similarity

Sites

Metal binding2981Magnesium or manganese 1 By similarity
Metal binding3111Magnesium or manganese 1 By similarity
Metal binding3111Magnesium or manganese 2 By similarity
Metal binding3131Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A8FA55 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: F436D4B60A45E6AD

FASTA36039,946
        10         20         30         40         50         60 
MKISLGLIYG GKSAEHNVSL QTALAVTKAL NTEKFDIHPI YITEEGEWLR GEKLTEPVSN 

        70         80         90        100        110        120 
VRMLQFEQNA KTFLPTSLNE SMFPQPTSAD EKIDVVFPLL HGPNGEDGTM QGLLELLNIP 

       130        140        150        160        170        180 
YVGNGVLASA AGMDKVMMKD VFAQAGLAQA KHLSFNKKDY EKATPDSLEQ VEQVLGYPCF 

       190        200        210        220        230        240 
VKPANMGSSV GISKCRSKEE LQTAFDLAFQ YDRRVVVEEG VVGREIEIGV LGNDEPKCSV 

       250        260        270        280        290        300 
VGEIAPKTDF YDYKAKYEDG DTDLIIPASV SEDEYKTIHD MAIKAFKSLD GSGLVRADFF 

       310        320        330        340        350        360 
LTEKGEVLIN EVNTMPGFTP FSMFPLLWKH TGVEYPELIE KLVSLAIERH QEKQTIKTTF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000813 Genomic DNA. Translation: ABV61122.1.
RefSeqYP_001485682.1. NC_009848.1.

3D structure databases

ProteinModelPortalA8FA55.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315750.BPUM_0427.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV61122; ABV61122; BPUM_0427.
GeneID5619654.
KEGGbpu:BPUM_0427.
PATRIC18964605. VBIBacPum16546_0438.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAKLAFQYD.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycBPUM315750:GH6N-444-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_BACP2
AccessionPrimary (citable) accession number: A8FA55
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways