Reviewed, UniProtKB/Swiss-Prot A8F9S1 (AROK_BACP2)

Names and origin

Protein names

Recommended name:
    Shikimate kinase
      Short name=SK
    EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	BPUM_0290

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity.
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	Shikimate kinase HAMAP MF_00109		PRO_1000094373
Regions
	Nucleotide binding	21 – 26	6	ATP By similarity
Sites
	Metal binding	25	1	Magnesium By similarity
	Binding site	43	1	Substrate By similarity
	Binding site	67	1	Substrate By similarity
	Binding site	90	1	Substrate; via amide nitrogen By similarity
	Binding site	129	1	ATP By similarity
	Binding site	147	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8F9S1-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 73C54CC5B47FB842	FASTA	185	21,920
	10 20 30 40 50 60 MKINREIPVR QRNIVLIGFM GVGKTTIGQL VAKKLYRDFI DVDQEIEKKY NMTIPEMFQQ 70 80 90 100 110 120 KGEAFFRQAE KDYIVDLCEH TQLKIVSLGG GAFKQEEIKR ACLKHCTVLF LDLSWENWKQ 130 140 150 160 170 180 RLDILIENRP VLHNRTLDEM KELFEERREI YSLHNSRVET DHLEAEEVAN YIVDTLKLGW DLYSK

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000813 Genomic DNA. Translation: ABV60988.1.
RefSeq	YP_001485548.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8F9S1.
Genome annotation databases
GeneID	5619517.
GenomeReviews	Gene locus BPUM_0290 in contig CP000813_GR.
KEGG	bpu:BPUM_0290.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IVESIKW.
Family and domain databases
HAMAP	MF_00109. [Tree]
InterPro	IPR000623. Shik_kinase. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_BACP2

Accession

Primary (citable) accession number: A8F9S1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)