Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Bacillus pumilus (strain SAFR-032)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of lactate to pyruvate.UniRule annotation

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Enzyme regulationi

Allosterically activated by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17NAD; via amide nitrogenUniRule annotation1
Binding sitei38NADUniRule annotation1
Binding sitei43NADUniRule annotation1
Binding sitei69NADUniRule annotation1
Binding sitei86SubstrateUniRule annotation1
Binding sitei92SubstrateUniRule annotation1
Binding sitei147NADUniRule annotation1
Binding sitei157Allosteric activatorUniRule annotation1
Binding sitei172Allosteric activatorUniRule annotation1
Active sitei179Proton acceptorUniRule annotation1
Binding sitei233SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi83 – 84NADUniRule annotation2
Nucleotide bindingi122 – 124NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Oxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciBPUM315750:G1G9U-322-MONOMER
UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenaseUniRule annotation (EC:1.1.1.27UniRule annotation)
Short name:
L-LDHUniRule annotation
Gene namesi
Name:ldhUniRule annotation
Ordered Locus Names:BPUM_0277
OrganismiBacillus pumilus (strain SAFR-032)
Taxonomic identifieri315750 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001355 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000604381 – 315L-lactate dehydrogenaseAdd BLAST315

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224PhosphotyrosineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiA8F9Q8

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi315750.BPUM_0277

Structurei

3D structure databases

ProteinModelPortaliA8F9Q8
SMRiA8F9Q8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 127Substrate bindingUniRule annotation4
Regioni152 – 155Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
HOGENOMiHOG000213793
KOiK00016
OMAiMDLMQTA
OrthoDBiPOG091H02EQ

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

A8F9Q8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNEKVNKVA LIGAGFVGSS YAFTLINQVI TDELVVIDLN QDKAMGDVMD
60 70 80 90 100
LNHGKAFAPH PVNTWYGDYE DCKDADIVCI CAGANQKPGE TRLDLVEKNL
110 120 130 140 150
NIFKGIVDNV MKSGFDGIFL VATNPVDILT YATWKFSGLP KERVIGSGTT
160 170 180 190 200
LDTARFRYML SEYFDAAAHN VHAYIIGEHG DTELAVWSHA NIGSVPITEL
210 220 230 240 250
MKKNDQYKQE DLDEMMENVR HAAYQIIEKK GATYYGVAMS LARITRAILH
260 270 280 290 300
NENSILTVST YLDGEYGADD VYIGVPALVN RNGATEVIEL ALNDTEKEQF
310
AHSVNVLKEI LAPHF
Length:315
Mass (Da):34,845
Last modified:November 13, 2007 - v1
Checksum:iD83F3444F7FBE3DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000813 Genomic DNA Translation: ABV60975.1
RefSeqiWP_012008847.1, NC_009848.4

Genome annotation databases

EnsemblBacteriaiABV60975; ABV60975; BPUM_0277
KEGGibpu:BPUM_0277

Similar proteinsi

Entry informationi

Entry nameiLDH_BACP2
AccessioniPrimary (citable) accession number: A8F9Q8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health