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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Bacillus pumilus (strain SAFR-032)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateUniRule annotation1
Binding sitei124NAD or substrateUniRule annotation1
Binding sitei155SubstrateUniRule annotation1
Active sitei179Proton acceptor1
Binding sitei233SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 43NADUniRule annotationAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenaseUniRule annotation (EC:1.1.1.27UniRule annotation)
Short name:
L-LDHUniRule annotation
Gene namesi
Name:ldhUniRule annotation
Ordered Locus Names:BPUM_0277
OrganismiBacillus pumilus (strain SAFR-032)
Taxonomic identifieri315750 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001355 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000604381 – 315L-lactate dehydrogenaseAdd BLAST315

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224PhosphotyrosineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiA8F9Q8.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi315750.BPUM_0277.

Structurei

3D structure databases

ProteinModelPortaliA8F9Q8.
SMRiA8F9Q8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213793.
KOiK00016.
OMAiMDLMQTA.
OrthoDBiPOG091H02EQ.

Family and domain databases

Gene3Di3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiView protein in InterPro
IPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiView protein in PROSITE
PS00064. L_LDH. 1 hit.

Sequencei

Sequence statusi: Complete.

A8F9Q8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNEKVNKVA LIGAGFVGSS YAFTLINQVI TDELVVIDLN QDKAMGDVMD
60 70 80 90 100
LNHGKAFAPH PVNTWYGDYE DCKDADIVCI CAGANQKPGE TRLDLVEKNL
110 120 130 140 150
NIFKGIVDNV MKSGFDGIFL VATNPVDILT YATWKFSGLP KERVIGSGTT
160 170 180 190 200
LDTARFRYML SEYFDAAAHN VHAYIIGEHG DTELAVWSHA NIGSVPITEL
210 220 230 240 250
MKKNDQYKQE DLDEMMENVR HAAYQIIEKK GATYYGVAMS LARITRAILH
260 270 280 290 300
NENSILTVST YLDGEYGADD VYIGVPALVN RNGATEVIEL ALNDTEKEQF
310
AHSVNVLKEI LAPHF
Length:315
Mass (Da):34,845
Last modified:November 13, 2007 - v1
Checksum:iD83F3444F7FBE3DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000813 Genomic DNA. Translation: ABV60975.1.
RefSeqiWP_012008847.1. NC_009848.4.

Genome annotation databases

EnsemblBacteriaiABV60975; ABV60975; BPUM_0277.
KEGGibpu:BPUM_0277.

Similar proteinsi

Entry informationi

Entry nameiLDH_BACP2
AccessioniPrimary (citable) accession number: A8F9Q8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: October 25, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families