A8F8Y5 (A8F8Y5_BACP2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit beta PIRNR PIRNR000804
EC=2.7.7.7 PIRNR PIRNR000804

Gene names

Name:	dnaN EMBL ABV60702.1
Ordered Locus Names:	BPUM_0002

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP] EMBL ABV60702.1

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus pumilus

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA By similarity. PIRNR PIRNR000804 SAAS SAAS022634
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). PIRNR PIRNR000804 SAAS SAAS022634
Subcellular location	Cytoplasm By similarity PIRNR PIRNR000804.

Ontologies

Keywords
Biological process	DNA replication PIRNR PIRNR000804 SAAS SAAS022634
Cellular component	Cytoplasm PIRNR PIRNR000804 SAAS SAAS022634
Molecular function	DNA-directed DNA polymerase PIRNR PIRNR000804 SAAS SAAS022634 EMBL ABV60702.1 Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	DNA polymerase III complex Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A8F8Y5 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 943380A2E2FB158A	FASTA	421	47,241
	10 20 30 40 50 60 MWIGCLSFLF TDLSTYSQAL LLLLRFLLIK YIYMSLRKFR RTTMKFTIQK DRLVESVQDV 70 80 90 100 110 120 LKAVSSRTTI PILTGIKIVA SDEGVSLTGS DSDISIESFI PQRDGDLEVI TIDRPGSIVL 130 140 150 160 170 180 QARFFSEIVK KLPMATVEIE VEQNHLTIIR SGSAEFNLNG LDAEEYPHLP QIEEHHAFQI 190 200 210 220 230 240 PTDLLKNLIR QTVFAVSTSE TRPILTGVNW KVEKGELICT ATDSHRLALR KAKLDIDEES 250 260 270 280 290 300 SYNVVIPGKS LTELSRILDD GQDLVSIVIT ETQVLFKAQN VLFFSRLLDG NYPDTARLIP 310 320 330 340 350 360 QESKTDVVVN TKEFLQAIDR ASLLAREGRN NVVKLSADPE QSLEISSNSP EIGKVVETVQ 370 380 390 400 410 420 ADDIKGEDLK ISFSPKYMLD ALKVLEGTEI HVSFTGAMRP FLLRTPNDDS ILQLILPVRT Y

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAFR-032.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000813 Genomic DNA. Translation: ABV60702.1.
RefSeq	YP_001485262.1. NC_009848.1.
3D structure databases
ProteinModelPortal	A8F8Y5.
ModBase	Search...
Protein-protein interaction databases
STRING	315750.BPUM_0002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV60702; ABV60702; BPUM_0002.
GeneID	5619212.
KEGG	bpu:BPUM_0002.
PATRIC	18963703. VBIBacPum16546_0002.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0592.
HOGENOM	HOG000071792.
KO	K02338.
OMA	DYNRVIP.
ProtClustDB	PRK05643.
Enzyme and pathway databases
BioCyc	BPUM315750:GH6N-95-MONOMER.
Family and domain databases
InterPro	IPR001001. DNA_polIII_beta. IPR022635. DNA_polIII_beta_C. IPR022637. DNA_polIII_beta_cen. IPR022634. DNA_polIII_beta_N. [Graphical view]
Pfam	PF00712. DNA_pol3_beta. 1 hit. PF02767. DNA_pol3_beta_2. 1 hit. PF02768. DNA_pol3_beta_3. 1 hit. [Graphical view]
PIRSF	PIRSF000804. DNA_pol_III_b. 1 hit.
SMART	SM00480. POL3Bc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00663. dnan. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A8F8Y5_BACP2

Accession

Primary (citable) accession number: A8F8Y5

Entry history

Integrated into UniProtKB/TrEMBL:	November 13, 2007
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)