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A8F8B7 (SYL_THELT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Tlet_1847
OrganismThermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO) [Complete proteome] [HAMAP]
Taxonomic identifier416591 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length815 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 815815Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000071114

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8F8B7 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: FE81700E65574478

FASTA81594,816
        10         20         30         40         50         60 
MPRYEPQEIE SKWQKFWEKE GLFKTPQHSD LPKYYMLVMF PYPSGTLHVG HVKNYVIGDA 

        70         80         90        100        110        120 
VARYKRMRAY NVLHPFGYDA FGLPAENAAI ERKIHPKDWT LNNINIIRRQ IKKLGISYDW 

       130        140        150        160        170        180 
NREVITCLES YYKWTEWIFL KLYEAGLAYK KKAAVNWCPK CMTSLANEQV KDGRCERCDT 

       190        200        210        220        230        240 
PVTIKHLEQW FFKITDYAER LLKDLDKLTG WPEHVKTMQR NWIGESKGAK ISFKVEESEM 

       250        260        270        280        290        300 
NIEVFTTRPD TLWGVTFMVL APESDLVNHI TLPELKGELE KFLNYVGQQD RHKRGSADVE 

       310        320        330        340        350        360 
KEGFFTGRYA INPVSGERIP IYVANYVLME YGTGAVMGVP AHDQRDYEFA VKYNLPIKEV 

       370        380        390        400        410        420 
IKPSEDIPKN RAYDGPGIMV NSGPLNGTVV PEGIERVIQW LEERGIAKRS VQYKLRDWLI 

       430        440        450        460        470        480 
SRQRYWGAPI PIVYCEKCGM VPVPEKSLPV QLPYDVEFLP TGQSPLMLND EFRKTSCPKC 

       490        500        510        520        530        540 
GGPALRDADT MDTFVDSSWY FLRYVNPDRD DVPFVKEDVD HWLPVDQYVG GVEHAVLHLL 

       550        560        570        580        590        600 
YSRFITKVLY DLGYLSFDEP FENLFTQGMI YKDGAKMSKS KGNVVSPDEM IDRYGADTLR 

       610        620        630        640        650        660 
MYILFMGPPE RDAEWNDAGI EGVYRFIRRA WSLMDQIISL PDNQNQVFAE EEKHLRRKLH 

       670        680        690        700        710        720 
ISLRKVTQDM EGGFKFNTVV SSLMELVNDA YDYIDNLPQH RWNIKLLKEL AKNFVLMISP 

       730        740        750        760        770        780 
FAPHFAEELW QRMGYSSSVM KEKWPEYDPD ALVVEEVEIA IQVNGKLRDK VKIPVDATED 

       790        800        810 
QIKEIALKSE KVRKYISDEP KKIIYVPKRL LNIIF 

« Hide

References

[1]"Complete sequence of Thermotoga lettingae TMO."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Bruce D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.
Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-301 / DSM 14385 / TMO.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000812 Genomic DNA. Translation: ABV34401.1.
RefSeqYP_001471465.1. NC_009828.1.

3D structure databases

ProteinModelPortalA8F8B7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING416591.Tlet_1847.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV34401; ABV34401; Tlet_1847.
GeneID5607865.
KEGGtle:Tlet_1847.
PATRIC23934397. VBITheLet125395_1933.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycTLET416591:GI64-1892-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_THELT
AccessionPrimary (citable) accession number: A8F8B7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 13, 2007
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries