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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Thermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei579 – 5791ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTLET416591:GI64-1892-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Tlet_1847
OrganismiThermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO)
Taxonomic identifieri416591 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000002016: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 815815Leucine--tRNA ligasePRO_1000071114Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi416591.Tlet_1847.

Structurei

3D structure databases

ProteinModelPortaliA8F8B7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionAdd
BLAST
Motifi576 – 5805"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8F8B7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRYEPQEIE SKWQKFWEKE GLFKTPQHSD LPKYYMLVMF PYPSGTLHVG
60 70 80 90 100
HVKNYVIGDA VARYKRMRAY NVLHPFGYDA FGLPAENAAI ERKIHPKDWT
110 120 130 140 150
LNNINIIRRQ IKKLGISYDW NREVITCLES YYKWTEWIFL KLYEAGLAYK
160 170 180 190 200
KKAAVNWCPK CMTSLANEQV KDGRCERCDT PVTIKHLEQW FFKITDYAER
210 220 230 240 250
LLKDLDKLTG WPEHVKTMQR NWIGESKGAK ISFKVEESEM NIEVFTTRPD
260 270 280 290 300
TLWGVTFMVL APESDLVNHI TLPELKGELE KFLNYVGQQD RHKRGSADVE
310 320 330 340 350
KEGFFTGRYA INPVSGERIP IYVANYVLME YGTGAVMGVP AHDQRDYEFA
360 370 380 390 400
VKYNLPIKEV IKPSEDIPKN RAYDGPGIMV NSGPLNGTVV PEGIERVIQW
410 420 430 440 450
LEERGIAKRS VQYKLRDWLI SRQRYWGAPI PIVYCEKCGM VPVPEKSLPV
460 470 480 490 500
QLPYDVEFLP TGQSPLMLND EFRKTSCPKC GGPALRDADT MDTFVDSSWY
510 520 530 540 550
FLRYVNPDRD DVPFVKEDVD HWLPVDQYVG GVEHAVLHLL YSRFITKVLY
560 570 580 590 600
DLGYLSFDEP FENLFTQGMI YKDGAKMSKS KGNVVSPDEM IDRYGADTLR
610 620 630 640 650
MYILFMGPPE RDAEWNDAGI EGVYRFIRRA WSLMDQIISL PDNQNQVFAE
660 670 680 690 700
EEKHLRRKLH ISLRKVTQDM EGGFKFNTVV SSLMELVNDA YDYIDNLPQH
710 720 730 740 750
RWNIKLLKEL AKNFVLMISP FAPHFAEELW QRMGYSSSVM KEKWPEYDPD
760 770 780 790 800
ALVVEEVEIA IQVNGKLRDK VKIPVDATED QIKEIALKSE KVRKYISDEP
810
KKIIYVPKRL LNIIF
Length:815
Mass (Da):94,816
Last modified:November 13, 2007 - v1
Checksum:iFE81700E65574478
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000812 Genomic DNA. Translation: ABV34401.1.
RefSeqiWP_012003877.1. NC_009828.1.
YP_001471465.1. NC_009828.1.

Genome annotation databases

EnsemblBacteriaiABV34401; ABV34401; Tlet_1847.
GeneIDi5607865.
KEGGitle:Tlet_1847.
PATRICi23934397. VBITheLet125395_1933.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000812 Genomic DNA. Translation: ABV34401.1.
RefSeqiWP_012003877.1. NC_009828.1.
YP_001471465.1. NC_009828.1.

3D structure databases

ProteinModelPortaliA8F8B7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi416591.Tlet_1847.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV34401; ABV34401; Tlet_1847.
GeneIDi5607865.
KEGGitle:Tlet_1847.
PATRICi23934397. VBITheLet125395_1933.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciTLET416591:GI64-1892-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-301 / DSM 14385 / TMO.

Entry informationi

Entry nameiSYL_THELT
AccessioniPrimary (citable) accession number: A8F8B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 13, 2007
Last modified: January 7, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.