A8F7M7 (A8F7M7_THELT) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	Tlet_1607

Organism

Thermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO) [Complete proteome] [HAMAP]

Taxonomic identifier

416591 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 SAAS SAAS003694 RuleBase RU004252
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS003694 HAMAP MF_00193 RuleBase RU003811 NAD HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	27 – 34	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	29	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	A8F7M7 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: F365B5B07909B8B1	FASTA	281	32,267
	10 20 30 40 50 60 MKNCIDEICE FISNFVKEYS YKGAVVGISG GLDSAVVAAL CVRALGAEKV FGLILPERDS 70 80 90 100 110 120 SKESVKDAKT VCENLKIHYE IKPATPMLRK LGIYKLFPPA FLFPKSVQER YVLGKWQKLS 130 140 150 160 170 180 NDPFIDDLLN KGNKEFLKGL AYYRSKHRIR MCILYFEAEK RNYAVVGTTN KTEFLTGLYV 190 200 210 220 230 240 KWGDDSVDIE PIAHLYKTQI FELARHLKIP EKILKKPPSP DLIPGITDEY AFGMSYQELD 250 260 270 280 RILQKIEQKK DLSGEPIEKV NRVIRILQAA KYREIKSFRI F

References

[1]

"Complete sequence of Thermotoga lettingae TMO." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Bruce D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-301 / DSM 14385 / TMO.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000812 Genomic DNA. Translation: ABV34161.1.
RefSeq	YP_001471225.1. NC_009828.1.
3D structure databases
ProteinModelPortal	A8F7M7.
ModBase	Search...
Protein-protein interaction databases
STRING	A8F7M7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5608814.
GenomeReviews	Gene locus Tlet_1607 in contig CP000812_GR.
KEGG	tle:Tlet_1607.
PATRIC	23933899. VBITheLet125395_1689.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	DGAVDCH.
ProtClustDB	CLSK875266.
Enzyme and pathway databases
BioCyc	TLET416591:TLET_1607-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01916.
Pfam	PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 2 hits.
ProtoNet	Search...

Entry information

Entry name

A8F7M7_THELT

Accession

Primary (citable) accession number: A8F7M7

Entry history

Integrated into UniProtKB/TrEMBL:	November 13, 2007
Last sequence update:	November 13, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)